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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer-Verlag

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DOI

10.1007/978-1-4471-4537-0_4

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1572-9818

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Suppression Subtractive Hybridization Identified D

Authors: Zaijun Yang Zhengsong Peng Hui Yang Jun Yang Shuhong Wei Peng Cai
Publish Date: 2010/09/03
Volume: 29, Issue: 2, Pages: 431-439
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Abstract

To understand the molecular mechanism of the three pistils mutation in wheat two forward subtractive cDNA libraries from two pairs of nearisogenic wheat lines Chuanmai 28 three pistils CMTP and Chinese Spring three pistils CSTP were constructed using SSH A total of 68 clones in CMTP lines and 197 clones in CSTP lines were identified as potentially overexpressed clones Thirtytwo out of 68 clones in CMTP lines belonged to unknown proteins while the remaining 30 clones shared homology to diverse classes of genes involved in protein modulation and protein synthesis signal transduction and ion transporters Approximately 67 of genes in CSTP lines were either unclassified or had no matches “no hits” in the database and about 33 of identified genes encoded polypeptides with known functions Sequence comparisons of cDNA clones between the two forward cDNA libraries revealed that four genes encoding thioredoxin H ubiquitin protein ligases MCM2 and ubiquinolcytochrome C reductase complex 14 kDa proteins were overexpressed in both libraries These genes would likely play an important role in determining the three pistils trait in the mutant wheat line

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