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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer-Verlag

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DOI

10.1007/978-3-642-68023-6

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1572-9818

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A Emphasis Type="Italic"pFBP6Barnase/Emphasis

Authors: Liao Liao CaiXian Liu GuoGui Ning ManZhu Bao
Publish Date: 2012/03/24
Volume: 30, Issue: 5, Pages: 1196-1203
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Abstract

The potential for transgene dispersal through pollen fruit and seed is an important argument against the release of genetically modified plants One approach toward addressing the concerns of gene flow from transgenic crops into closely related wild species involves in the use of tissuespecific promoters to engineer male and/or female sterility In this study we investigated the potential of Barnase ectopic expression for engineering floral sterility A 26 kb promoter region of floral binding protein 6 FBP6 from Petunia hybrida was isolated and fused to a reporter gene encoding βglucuronidase GUS The construct was introduced into tobacco plants where GUS staining was detected ubiquitously throughout the various tissues The expression pattern of FBP6 resembled AG promoters ie weak promoter activity was found in vegetative tissues and strong activity was found in the various floral organs including the carpels and stigma MeanwhileThe pFBP6Barnase construct was then cotransformed into tobacco along with the Barstar gene encoding an enzymatic inhibitor of Barnase which was expressed at low but ubiquitous levels Although cotransformed tobacco plants showed near normal vegetative growth 74 of transgenic plants exhibited stigma and style ablation and 98 of flower buds abscised before opening Further analyses confirmed that stigma and style ablation prevented fertilization of the flower and abscission of the bud followed rapidly Thus this approach has advantages for those ornamental/landscaping species where the pollen and fruit represent pollutants of the urban environment eg platanus and poplar

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Other Papers In This Journal:
  1. Genome-Wide Identification of microRNAs and Their Targets in Cold-Stored Potato Tubers by Deep Sequencing and Degradome Analysis
  2. An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides
  3. Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat
  4. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  5. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  6. Transcriptional Regulation of the Rice Phytase Gene OsPHY1 by Several Phytohormones and Osmotic Stresses Using Promoter-GUS Analysis
  7. Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels
  8. Wheat Neocentromeres Found in F1 Triticale × Tritordeum Hybrids (AABBRH ch ) After 5-Azacytidine Treatment
  9. BcJMJ30 , the Gene Encoding jmjC Domain-Containing Histone Demethylase Is Associated with Pollen Development and Fertilization in Brassica campestris ssp. chinensis
  10. Differential Protein Phosphorylation Regulates Chloroplast Movement in Response to Strong Light and Darkness in Arabidopsis thaliana
  11. Sulfite Oxidase is Essential for Timely Germination of Maize Seeds upon Sulfite Exposure
  12. Molecular Cloning, Biochemical Characterization, and Differential Expression of an Acetyl-CoA C-Acetyltransferase Gene ( AACT ) of Brahmi ( Bacopa monniera )
  13. Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco
  14. Enhanced cloning efficiency of differential display PCR amplicons
  15. Virus-Induced Gene Silencing of a Putative Glutathione S -Transferase Gene Compromised Ol-1 -Mediated Resistance against Powdery Mildew in Tomato
  16. Flow Karyotyping of Wheat Addition Line “T240” with a Haynaldia villosa 6VS Telosome
  17. Expression Analysis of Fatty Acid Biosynthetic Pathway Genes during Interactions of Oil Palm ( Elaeis guineensis Jacq.) with the Pathogenic Ganoderma boninense and Symbiotic Trichoderma harzianum Fungal Organisms
  18. Small RNA and Degradome Deep Sequencing Reveals Peanut MicroRNA Roles in Response to Pathogen Infection
  19. Isolation and amplification of DNA from rhizomes of turmeric and ginger
  20. A Quantitative Method to Monitor the Efficacy of Inhibitors Against the Chymotrypsin-Like Activity of the Proteasome in Tobacco Leaf Protoplasts
  21. Phylogenetic Analysis of S -Locus Genes Reveals the Complicated Evolution Relationship of S Haplotypes in Brassica
  22. QTL Mapping of Yield and Yield Components under Normal and Salt-stress Conditions in Bread Wheat ( Triticum aestivum L. )
  23. Expression Profile of Early Responsive Genes Under Salt Stress in Upland Cotton ( Gossypium hirsutum L.)
  24. A Novel Co-immunoprecipitation Protocol Based on Protoplast Transient Gene Expression for Studying Protein–protein Interactions in Rice
  25. Cloning and Expression Analysis of Wheat Pheophorbide a Oxygenase Gene TaPaO
  26. Expression of Aprotinin in Anther Causes Male Sterility in Tobacco var Petit havana
  27. Highly Conserved UFD1 Proteins Among Eukaryotes Exhibit Considerable C-Terminus Diversity in Different Taxa
  28. Isolation of cDNAs encoding for two distinct isoforms of the TATA-Box binding proteins from common bean ( Phaseolus vulgaris L.)
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