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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer US

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DOI

10.1002/chin.200102134

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ISSN

1572-9818

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GenomeWide Identification of microRNAs and Their

Authors: Yongbin Ou Xun Liu Conghua Xie Huiling Zhang Yuan Lin Meng Li Botao Song Jun Liu
Publish Date: 2014/08/12
Volume: 33, Issue: 3, Pages: 584-597
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Abstract

Potato tubers the economically important organ for food processing raw materials and seeds are usually stored at different temperatures for various purposes A general physiological process during storage is starch hydrolysis and accumulation of reducing sugars as breaking of tuber dormancy The starch hydrolysis can be accelerated by low temperature Valuable works have been reported on transcriptional and posttranslational regulatory mechanisms that are involved in starch hydrolysis in stored potato tubers However little is known whether posttranscriptional events are also involved in this important process In the present study two small RNA libraries and two degradome libraries were constructed using 4 and 20 °Cstored potato tubers Deep sequencing and whole genomewide data analysis led to the identification of 53 known miRNAs and 60 novel miRNAs largely increasing the number of miRNAs known in potato Seventy genes were identified as miRNA targets by degradome analysis indicating that miRNAs were active in gene expression regulation in the postharvested tubers The targets were potentially involved in various biological processes such as stimulus response signal transduction transcriptional regulation and metabolism Further expression profiling revealed 11 miRNAs/miRNAs and 34 targets that showed different patterns between two potato genotypes with distinct response to cold storage in terms of reducing sugar accumulation implying a role of miRNAs in coldinduced sweetening This study is the first genomewide investigation of miRNAs and their targets in coldstored potato tubers thus broadening our perspectives on the roles of miRNAs in cold response dormancy and carbohydrate metabolismWe would like to thank Prof Feng Li for critically reading the manuscript This study was supported by the National Natural Science Foundation of China 31171602 and 31201258 the Recommend International Advanced Agricultural Science and Technology 948 Program of China 2014Z23 and the Fundamental Research Funds for the Central Universities of China XDJK2014B016

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