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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer-Verlag

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DOI

10.1002/sim.5538

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1572-9818

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Enhanced cloning efficiency of differential displa

Authors: Natasha M Sanabria Ian A Dubery
Publish Date: 2012/09/03
Volume: 22, Issue: 3, Pages: 309-309
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Abstract

Differential display offers a rapid and simultaneous comparison of expression profiles of mRNA from variant cells having the same genetic background An inherent flaw of the technique may yield falsepositives on subsequent analyses The only source of the band of interest is the denaturing sequencing gel from which the band is identified Consequently the integrity of the fragment is compromised and cloning required to gain sequence information becomes costly and timeconsuming Described herein are modifications used to stabilize the integrity of the fragment as well as to increase the number of transcripts cloned Additional steps were added to the processing of the differential display reverse transcriptionpolymerase chain reaction DDRTPCR fragment and cloning reactions were either adapted to concentrate the product or specifically optimized at certain points to prompt efficiency Therefore the overall cost and time spent analyzing truepositives as confirmed by reverse Northern blots was reduced

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  2. An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides
  3. Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat
  4. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  5. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  6. Transcriptional Regulation of the Rice Phytase Gene OsPHY1 by Several Phytohormones and Osmotic Stresses Using Promoter-GUS Analysis
  7. Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels
  8. Wheat Neocentromeres Found in F1 Triticale × Tritordeum Hybrids (AABBRH ch ) After 5-Azacytidine Treatment
  9. BcJMJ30 , the Gene Encoding jmjC Domain-Containing Histone Demethylase Is Associated with Pollen Development and Fertilization in Brassica campestris ssp. chinensis
  10. Differential Protein Phosphorylation Regulates Chloroplast Movement in Response to Strong Light and Darkness in Arabidopsis thaliana
  11. Sulfite Oxidase is Essential for Timely Germination of Maize Seeds upon Sulfite Exposure
  12. Molecular Cloning, Biochemical Characterization, and Differential Expression of an Acetyl-CoA C-Acetyltransferase Gene ( AACT ) of Brahmi ( Bacopa monniera )
  13. Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco
  14. A pFBP6::Barnase Construct Resulted in Stigma and Style Ablation and Floral Abscission in Transgenic Tobacco
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  20. A Quantitative Method to Monitor the Efficacy of Inhibitors Against the Chymotrypsin-Like Activity of the Proteasome in Tobacco Leaf Protoplasts
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  22. QTL Mapping of Yield and Yield Components under Normal and Salt-stress Conditions in Bread Wheat ( Triticum aestivum L. )
  23. Expression Profile of Early Responsive Genes Under Salt Stress in Upland Cotton ( Gossypium hirsutum L.)
  24. A Novel Co-immunoprecipitation Protocol Based on Protoplast Transient Gene Expression for Studying Protein–protein Interactions in Rice
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  26. Expression of Aprotinin in Anther Causes Male Sterility in Tobacco var Petit havana
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