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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer-Verlag

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DOI

10.1007/s00268-016-3529-7

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1572-9818

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An improved RNA isolation method for succulent pla

Authors: H H Gehrig K Winter J Cushman A Borland T Taybi
Publish Date: 2012/09/03
Volume: 18, Issue: 4, Pages: 369-376
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Abstract

Isolation of high quality high molecular weight RNA from plant tissues is complicated by high levels of polyphenols and polysaccharides which bind to and/or coprecipitate with RNA Using high molecular weight polyethylene glycol HMWPEG RNA was successfully isolated from plant species in which other RNA extraction methods and commercially available kits failed to deliver suitable results We tested various buffer systems and isolation conditions with and without PEG or PVP polyvinylpyrrolidone using tissue from species ofAloe Ananas Clusia Euphorbia Kalanchoe Opuntia andPyrrosia all of which contain high amounts of phenolic compounds and/or polysaccharides HMWPEG was found to be more effective than PVP in removing these compounds RNA extraction using HMWPEG resulted in RNA of high quality from all species investigated as indicated by UV light absorption profiles and also yielded PCR amplification products after reverse transcription

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Other Papers In This Journal:
  1. Genome-Wide Identification of microRNAs and Their Targets in Cold-Stored Potato Tubers by Deep Sequencing and Degradome Analysis
  2. Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat
  3. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  4. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  5. Transcriptional Regulation of the Rice Phytase Gene OsPHY1 by Several Phytohormones and Osmotic Stresses Using Promoter-GUS Analysis
  6. Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels
  7. Wheat Neocentromeres Found in F1 Triticale × Tritordeum Hybrids (AABBRH ch ) After 5-Azacytidine Treatment
  8. BcJMJ30 , the Gene Encoding jmjC Domain-Containing Histone Demethylase Is Associated with Pollen Development and Fertilization in Brassica campestris ssp. chinensis
  9. Differential Protein Phosphorylation Regulates Chloroplast Movement in Response to Strong Light and Darkness in Arabidopsis thaliana
  10. Sulfite Oxidase is Essential for Timely Germination of Maize Seeds upon Sulfite Exposure
  11. Molecular Cloning, Biochemical Characterization, and Differential Expression of an Acetyl-CoA C-Acetyltransferase Gene ( AACT ) of Brahmi ( Bacopa monniera )
  12. Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco
  13. A pFBP6::Barnase Construct Resulted in Stigma and Style Ablation and Floral Abscission in Transgenic Tobacco
  14. Enhanced cloning efficiency of differential display PCR amplicons
  15. Virus-Induced Gene Silencing of a Putative Glutathione S -Transferase Gene Compromised Ol-1 -Mediated Resistance against Powdery Mildew in Tomato
  16. Flow Karyotyping of Wheat Addition Line “T240” with a Haynaldia villosa 6VS Telosome
  17. Expression Analysis of Fatty Acid Biosynthetic Pathway Genes during Interactions of Oil Palm ( Elaeis guineensis Jacq.) with the Pathogenic Ganoderma boninense and Symbiotic Trichoderma harzianum Fungal Organisms
  18. Small RNA and Degradome Deep Sequencing Reveals Peanut MicroRNA Roles in Response to Pathogen Infection
  19. Isolation and amplification of DNA from rhizomes of turmeric and ginger
  20. A Quantitative Method to Monitor the Efficacy of Inhibitors Against the Chymotrypsin-Like Activity of the Proteasome in Tobacco Leaf Protoplasts
  21. Phylogenetic Analysis of S -Locus Genes Reveals the Complicated Evolution Relationship of S Haplotypes in Brassica
  22. QTL Mapping of Yield and Yield Components under Normal and Salt-stress Conditions in Bread Wheat ( Triticum aestivum L. )
  23. Expression Profile of Early Responsive Genes Under Salt Stress in Upland Cotton ( Gossypium hirsutum L.)
  24. A Novel Co-immunoprecipitation Protocol Based on Protoplast Transient Gene Expression for Studying Protein–protein Interactions in Rice
  25. Cloning and Expression Analysis of Wheat Pheophorbide a Oxygenase Gene TaPaO
  26. Expression of Aprotinin in Anther Causes Male Sterility in Tobacco var Petit havana
  27. Highly Conserved UFD1 Proteins Among Eukaryotes Exhibit Considerable C-Terminus Diversity in Different Taxa
  28. Isolation of cDNAs encoding for two distinct isoforms of the TATA-Box binding proteins from common bean ( Phaseolus vulgaris L.)
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