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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer-Verlag

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DOI

10.1016/0165-6147(89)90267-8

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1572-9818

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Detection of single nucleotide mutations in wheat

Authors: P MartinsLopes H Zhang R Koebner
Publish Date: 2012/09/03
Volume: 19, Issue: 2, Pages: 159-162
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Abstract

We show that single strand conformation polymorphism SSCP analysis using the mutation detection enhancement MDETM matrix is efficient at detecting sequence polymorphisms in PCR amplicons Four independent wheat genomic fragments were amplified from two contrasting templates and sequenced The allelic fragments were differentiated at 1–6 single nucleotide positions but MDESSCP was able to unequivocally distinguish each allelic pair The approach is therefore considered a powerful way of identifying single nucleotide polymorphisms SNPs without extensive amplicon sequencing

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