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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer US

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ISSN

1572-9818

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Small RNA and Degradome Deep Sequencing Reveals Pe

Authors: Chuanzhi Zhao Han Xia Tingjie Cao Yu Yang Shuzhen Zhao Lei Hou Ye Zhang Changsheng Li Xinyou Zhang Xingjun Wang
Publish Date: 2014/10/11
Volume: 33, Issue: 4, Pages: 1013-1029
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Abstract

Bacterial wilt BW disease is a widespread disease in peanut Arachis hypogaea L reducing peanut production because of high susceptibility of most cultivated species However Arachis glabrata a perennial wild relative has high resistance to soilborne diseases MicroRNAs miRNAs have been characterized as important factors in plant growth development and biotic/abiotic stress responses However their roles in response to pathogen are still unknown in peanut Here we constructed four miRNA libraries from A hypogaea and A glabrata and identified 155 known miRNAs 31 novel miRNAs and 173 plausible candidates of novel miRNAs After infected with BW the expression of many known miRNA was induced or inhibited in both cultivated and wildtype peanuts Many miRNAs displayed differential expression patterns in these two species and the expression of some miRNAs was confirmed by quantitative realtime PCR qRTPCR analysis A total of 309 targets were predicted among which 74 of them were confirmed by degradome sequencing More than 10  of the targets were defense response genes such as hypersensitiveinduced response protein leucinerich repeat LRR receptorlike serine/threonineprotein kinase GRAS aquaporin lipid transfer protein ARF MYB transcription factors and MLPlike protein Our results indicate that diverse set of miRNAs of cultivated and wild peanut species is responsive to BW infection and suggests a possible role in peanut disease resistanceThis work is supported by the National Natural Science Foundation of China 31101427 grants from the Initial Special Research for 973 Program of China 2012CB126313 Ministry of Science and Technology of China 2013AA102602 2011BAD35B04 2012BAD33B07 Shandong Province Taishan Scholar Foundation tshw20100416 Shandong Province Germplasm Innovation and Utilization Project and grants from Shandong Province ZR2010CZ002 ZR2010CQ008 201103023 Young Talents Training Program of Shandong Academy of Agricultural SciencesXW and XZ designed the study CZ HX TC YY SZ LH YZ and CL carried out most of the experiments and data analysis and wrote the material and method part of the manuscript XW and XZ wrote the manuscript made the figures and finalized the tables

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