Effects and mechanisms of the functional parts of

Authors: Na Liu Juntian Liu Yuanyuan Ji Peipei Lu

Publish Date: 2012/04/11

Volume: 19, Issue: 6, Pages: 432-438

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Abstract

To investigate and compare the effects and mechanisms of three functional parts of Dahuang Zhechong Pill 大黄 Open image in new window 虫丸 DHZCP including drugs with the function of removing blood stasis and promoting blood circulation FPI drugs with the function of expelling heat and moistening dryness FPII and drugs with the function of nourishing yin and replenishing blood FPIII of DHZCP on plateletderived growth factor PDGFstimulated vascular smooth muscle cells VSMCs proliferation with the method of serum pharmacologyVSMCs proliferation of rat was assayed by measuring the cell viability with the 34 5dimethylthiazol2yl2 5diphenyltetrazolium bromide MTT method DNA synthesis in VSMCs was examined by detecting 5′bromo2′deoxyuridine incorporation with the immunocytochemical method Cycle of VSMCs was evaluated with flow cytometry Expression of cyclin D1 p27 PKCα and phosphorylated extracellular signal regulated kinase 1/2 ERK1/2 was quantified by the Western blotting methodThe FPI and FPIII containing serum was capable of inhibiting PDGFstimulated proliferation and DNA synthesis of VSMCs arrested VSMCs in G phase downregulated cyclin D1 and upregulated p27 expression P 001 or P 005 The FPI and FPIII containing serum also inhibited the PDGFinduced phosphorylation of tyrosine of ERK1/2 and PKCα expression P 001 or P 005FPI and FPIII of DHZCP are able to inhibit VSMCs proliferation via interrupting PKCαERK1/2 signaling modulating the expression of cell cycle proteins to result in arresting the cells in G phase The inhibitory effect is mainly related to the function of removing blood stasis and promoting blood circulation slightly to the function of nourishing yin and replenishing blood but not to the function of expelling heat and moistening dryness

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