Authors: Jing Cai Teng Zheng Lei Zhang Yun Tian Minhe Yang Jian Du
Publish Date: 2013/03/26
Volume: 17, Issue: 10, Pages: 758-763
Abstract
To comparatively analyze the difference in the expression of 54 transcription factors in the hypothalamus using protein chips following the medication of Chinese drugs for Shen 肾tonification Herba Epimedii and Fructus Ligustri lucidi FL to aged ratsWistar rats aged 15 months of SPF grade were randomized into three groups three males and three females in each group They were medicated with Herba Epimedii decoction HED 014 g/kg Fructus Ligustri lucidi decoction FLD 012 g/kg and distilled water respectively twice a day for 15 days The rats were sacrificed at the morning of the 16th day 1 h after medication and their hypothalamus was taken and made into homogenate under an icebath for detecting the expression of transcription factors with chip techniqueThe expressions of signal transduction and transcription activation factor6 Stat6 and androgen receptor AR were upregulated and those of preB transcription factor1 Pbx1 stat1 and AP2 were downregulated in both HED and FLD treated groups but these changes occurred mainly in female rats in the former while mainly in males in the latterChinese drugs for Shentonification could impact the expression of transcription factors in the hypothalamus of aged rats dominantly on the neuroendocrine factors responsible for the growth and development The effects of drugs for tonifying Shenyang and for tonifying Shenyin are different which is probably one of the pharmacological mechanisms of the Shentonifying drugs
Keywords: