Authors: Mingzheng Xie Qinghui Qi Shenglin Zhang Mingming Wei
Publish Date: 2015/04/29
Volume: 21, Issue: 8, Pages: 624-629
Abstract
Fifty Wistar rats were randomly assigned to the control group MODS model group and DCQD treated group The rats in MODS model group and DCQD treated group were injected Escherichia coli E coli suspension into abdominal cavity under sterile condition The DCQD treated group was gavaged with DCQD 2 days before the E coli suspension was injected Twentyfour hours after injection the proximal segment of intestine was resected and studied by immunohistofluorescence using vesicular acetylcholine transporter vasoactive intestinal polypeptide VIP substance P SP and neuronal nitric oxide synthase nNOS antibodies The wholemount preparations were observed by laser scanning confocal microscope to detect the changes of quantity and fluorescence integral optical density IOD value of intestine enteric nervesCompared with the control group the quantity and IOD value of acetylcholine ACh VIP SP and nitric oxide NO nerves of intestine in the MODS group were significantly decreased P001 and the network of enteric nerves was remarkably disrupted Compared with the MODS group the quantity and fluorescence IOD value of ACh VIP SP and NO nerves in the DCQD group were significantly increased P001 and the network of enteric nerves was remarkably recovered
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