Authors: Yuanlong Xie Min Zhou Huihao Ma Xiang Wang Juju Liu
Publish Date: 2015/05/09
Volume: 21, Issue: 12, Pages: 944-948
Abstract
A perfusion model of isolated thoracic aorta rings of rats was applied The effect of cumulative gastrodin 5 50 100150 200 and 250 μmol/L on endotheliumintact aorta rings was investigated The same procedure was applied to observe the effect of gastrodin on endotheliumintact/denuded aorta rings precontracted with 106 mol/L phenylephrine hydrochloride PE The aorta rings incubated by 200 mmol/L gastrodin in the Ca2+free KH solution was contracted by using PE The effect of 200 mmol/L gastrodin on endotheliumdenuded aorta rings precontracted with 60 mmol/L KCl was also observedCompared with the denuded gastrodin group the intact gastrodin group could significantly relax the PEcontracted aorta rings P001 In Ca2+free KH solution KHS the PEinduced contraction rate of aorta rings preincubated by gastrodin was 65±07 which was significantly less than the control group 118±09P001 However after 3 mmol/L CaCl2 was added the Ca2+induced contraction in the gastrodin group 517±24 was similar to that in the control group 498±28 The contractile rate of rings in the KClcontracted gastrodin group 963±06 was not significantly different from that in the control group 968±12Gastrodin has the effect of vasorelaxation on isolated thoracic aorta rings of rats The mechanism of the vasorelaxation of gastrodin may mainly work through the inhibition of inositol 1 4 5trisphosphosphate receptor on the sarcoplasmic reticulum of the arterial smooth muscle which leads to the reduction of the Ca2+ released from the sarcoplasmic reticulum
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