Authors: Jue Song Jun Li Shirui Zheng Yong Jin Yan Huang
Publish Date: 2013/03/15
Volume: 19, Issue: 5, Pages: 353-359
Abstract
A rat chronic bronchitis CB model was developed using lipopolysaccharide LPS combined with bacillus Calmette Guerin BCG YPF simple recipe Astragalus membranaceus Fisch Bge AM and Astragalus membranaceus Fisch Bge plus rhizome of Atractylodes macrocephala Koidz AM+RA decoction were administered intragastric administration once a day for 21 days to rats to prevent and treat CB Immunoregulatory and antiinflammatory effects of YPF AM and AM+RA were tested by serum pharmacology in vitro on splenic lymphocytes of normal rats and alveolar macrophages of CB ratsInflammation in the pulmonary tissue and the bronchus of CB rats was significantly reduced in the YPFtreatment groups AM and AM+RA groups demonstrating the efficacy of YPF Serum samples collected at different times from rats after administration of YPF AM and AM+RA demonstrated increased proliferation of splenic lymphocytes with area under the effect curve AUE of 5526 3363 and 4520 respectively Treatment of alveolar macrophages with serum samples in YPF AM or AM+RA group inhibited interleukin8 IL8 in the cell culture media and the effect was much better in the YPF group compared with AM or AM+RA group with a higher maximal effect Emax P005 and larger AUE P 001 and P005 Moreover serum from rats treated with AM or AM+RA had similar efficacy while the efficiency was lower than that treated with YPF
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