Journal Title
Title of Journal: Chin J Integr Med
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Abbravation: Chinese Journal of Integrative Medicine
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Publisher
Springer Berlin Heidelberg
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Authors: Lina Ma Lianda Li Shaochun Li Xinmei Hao Jinyan Zhang Ping He Yikui Li
Publish Date: 2016/07/13
Volume: 23, Issue: 8, Pages: 589-597
Abstract
Ninetyfour Wistar rats were randomly assigned to 6 groups n=14–16 per group sham control group underwent thoracotomy without left anterior descending LAD occlusion and only received an injection of the same amount of citrate buffer MI control group subjected to LAD occlusion and only received an injection of same amount of citrate buffer positive control group subjected to LAD occlusion and received an injection of diltiazem hydrochloride at the dose of 15 mg/kg and MI + allicin groups subjected to LAD occlusion and received an injection of allicin at the doses of 12 18 and 36 mg/kg All of the drugs were administered intraperitoneally daily for 21 days The infarct area was measured by myocardial staining Hematoxylineosin staining was used to observe the pathological changes Cardiac function parameters were assessed by echocardiography The myocardial apoptotic index was estimated by terminal deoxynucleotidyl transferasemediated dUTP nickend labeling staining The expression of Bax and Bcl2 were detected by quantificational realtime polymerase chain reaction and Western blotTreatment with allicin could attenuate the myocardial infarct area P005 and relieve the changes of the myocardium The left ventricular anterior wall diastolic and systolic thicknesses were increased in the allicintreated groups P005 while there was no signifificant difference in the left ventricular posterior wall diastolic and systolic thickness P005 The left ventricular internal diameter in systole ejection fraction fractional shortening and stroke volume were dramatically elevated in allicintreated rats P005 Allicin dosedependently reduced creatine kinase and lactate dehydrogenase levels P005 The myocardial apoptotic index was also markedly lowered and Bax expression was signifificantly decreased whereas Bcl2 expression exhibited an opposite trend in allicintreated rats P005We thank Prof Chen J Xinjiang Ailexin Parmaceutical Co Ltd China Prof Chen SK Xinjiang Ailexin Parmaceutical Co Ltd China and Prof Li XX Central Lab Xinjiang Medical University China for providing technical assistance and materials
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