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Title of Journal: Int J Legal Med

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Abbravation: International Journal of Legal Medicine

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Springer-Verlag

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DOI

10.1016/0002-9416(54)90111-6

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1437-1596

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A validation study of the Nucleix DSISemen kit—a

Authors: Bobby L LaRue Jonathan L King Bruce Budowle
Publish Date: 2012/08/16
Volume: 127, Issue: 2, Pages: 299-308
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Abstract

The detection of semen can assist in reconstructing the events of a sexual assault and impact the outcome of legal dispositions Many methods currently are used for detecting the presence of semen but they all have limitations with regards to specificity sample degradation/consumption stability of biomolecule assayed and/or incompatibility with downstream individual identification assays DNA is routinely collected at sexual assault crime scenes and is widely used for individual identification The DNA also carries methylation patterns that are tissue specific To date however assays designed to exploit methylation patterns suffer from complex chemistries and unwieldy analyses DSISemen™ kit uses a novel approach involving CpG methylationsensitive restriction endonuclease digestion coupled to a multiplexed polymerase chain reaction PCR to generate an amplicon profile that makes it possible to determine whether the tissue source of a DNA sample was semen or nonsemen The assay returned an appropriate positive result for semen with neat semen semen stains and semen/nonsemen tissue mixtures The assay is robust and reliable with a positive result for semen given as little as 31 pg of template DNA input Low levels of semen were detected in mixtures of semen and other body fluids UVexposed samples and those in the presence of limited concentrations of known PCR inhibitors were typeable The DSISemen™ kit provides a reliable tool for the determination of DNA being derived from semenThis work was supported in part by the IC Postdoctoral Research Fellowship program We also especially thank Adam Wasserstrom and Dan Frumkin of Nucleix Tel Aviv Israel for providing reagents software and technical expertise Special thanks are also in order for Christina Capt of the UNT Human Identification Forensic Laboratory for presumptive and confirmatory testing of samples reported in this study We also greatly appreciate Blake Meyers and Pam Marshall UNTHSC and Dr James Derr Texas AM University College of Veterinary Medicine for helping us acquire samples for species testingThe work described above was performed in accordance with all laws both Federal and State that apply to research researcher conduct and the protection of human test subjects We also are operating under the guidance of and in accordance with policies of the UNTHSC Institutional Review Board

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  2. Identification of human DNA in forensic evidence by loop-mediated isothermal amplification combined with a colorimetric gold nanoparticle hybridization probe
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