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Title of Journal: Int J Legal Med

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Abbravation: International Journal of Legal Medicine

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Springer Berlin Heidelberg

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DOI

10.1007/bf00162031

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1437-1596

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Diagnosis of aluminum phosphide poisoning using a

Authors: Hui Yan Ping Xiang Sujing Zhang Baohua Shen Min Shen
Publish Date: 2017/02/27
Volume: 131, Issue: 4, Pages: 1001-1007
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Abstract

Aluminum phosphide AlP is an effective and cheap pesticide that is commonly used worldwide but it is also a common cause of human poisoning and carries a high mortality rate AlP reacts with moisture in air water and hydrochloric acid in the stomach to produce phosphine PH3 gas Two routes of exposure are ingestion of AlP and inhalation of phosphine generated by the action of moisture on AlP Absorbed phosphine is rapidly metabolized into phosphite and hypophosphite A method is described for the analysis of the phosphine metabolites in various biological matrices The method involves reacting the sample with zinc and aqueous H2SO4 in a volatile organic analysis vial The metabolites were transformed into phosphine gas and then analyzed by headspace gas chromatography coupled with mass spectrometry HSGCMS This method is capable of detecting quantities of PH3 as low as 02 μg/mL in a sample After validation the method was applied to animal experiments and a real case of human AlP intoxication This approach has the advantage of detecting metabolites of PH3 in case the PH3 was converted and can be considered a useful additional tool for the diagnosis of AlP poisoning in forensic scienceThis work was supported by Ministry of Science and Technology 201 6YFC08007 04 the National Natural Science Foundation of China 81302614 the Science and Technology Commission of Shanghai Municipality 13ZR1443000 15DZ120750014DZ2270800/16DZ2290900 Ministry of Finance PR China GY2017G2 GY2015G3

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