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Title of Journal: Int J Legal Med

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Abbravation: International Journal of Legal Medicine

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Springer Berlin Heidelberg

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DOI

10.1016/0142-9612(87)90102-5

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ISSN

1437-1596

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Response of forest soil euglyphid testate amoebae

Authors: Christophe V W Seppey Bertrand Fournier Ildikò Szelecz David Singer Edward A D Mitchell Enrique Lara
Publish Date: 2015/01/27
Volume: 130, Issue: 2, Pages: 551-562
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Abstract

Decomposing cadavers modify the soil environment but the effect on soil organisms and especially on soil protists is still poorly documented We conducted a 35month experiment in a deciduous forest where soil samples were taken under pig cadavers control plots and fake pigs bags of similar volume as the pigs We extracted total soil DNA amplified the SSU ribosomal RNA rRNA gene V9 region and sequenced it by Illumina technology and analysed the data for euglyphid testate amoebae Rhizaria Euglyphida a common group of protozoa known to respond to microenvironmental changes We found 51 euglyphid operational taxonomic units OTUs 45 of which did not match any known sequence Most OTUs decreased in abundance underneath cadavers between days 0 and 309 but some responded positively after a time lag We sequenced the fulllength SSU rRNA gene of two common OTUs that responded positively to cadavers a phylogenetic analysis showed that they did not belong to any known euglyphid family This study confirmed the existence of an unknown diversity of euglyphids and that they react to cadavers Results suggest that metabarcoding of soil euglyphids could be used as a forensic tool to estimate the postmortem interval PMI particularly for longterm 2 months PMI for which no reliable tool existsThis study was funded by the Stiftung Forensisches Forum Frankfurt/Main Germany Vereinigung von Freunden und Förderern der GoetheUniversität Frankfurt/Main Germany the University of Neuchâtel Switzerland and the Swiss National Science Foundation project No 31003A 141188 to EM We thank Jan Boni forest engineer of Neuchâtel for providing the permission to use the site Dr Sonia Estelle Tarnawski and Luc Dolivo for the help with DNA extraction Dr Roxane KohlerMilleret for infrared spectroscopy and Emanuela Samaritani for the assistance with the Illumina sequencing We also thank Balazs Laurenczy for the constructive discussion on the informatics part

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