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Title of Journal: Int J Legal Med

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Abbravation: International Journal of Legal Medicine

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Springer-Verlag

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10.1007/11863908_11

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1437-1596

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Identification of forensically important Emphasis

Authors: Kurt Jordaens Gontran Sonet René Richet Erena Dupont Yves Braet Stijn Desmyter
Publish Date: 2012/09/08
Volume: 127, Issue: 2, Pages: 491-504
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Abstract

The identification of species of the forensically important genus Sarcophaga is very difficult and requires strong taxonomic expertise In this study we sequenced the mitochondrial cytochrome c oxidase subunit I COI gene of 126 specimens of 56 W European Sarcophaga species and added GenBank data to our database to yield a total dataset of 270 COI sequences from 99 Sarcophaga species to evaluate the COI gene as a molecular diagnostic tool for species identification in this genus Using two simple criteria Best Match BM and Best Close Match BCM we showed that the identification success using a minibarcode region of 127 bp was very low 807–825  and the use of this region is not recommended as a species identifier In contrast identification success was very high using the standard barcode region 658 bp or using the entire COI region 1535 bp 982–993  Yet there was a low interspecific sequence divergence 2  in six species groups so that for 16 out of the 99 species nine of which are of forensic importance the use of COI barcodes as species identifier should be done with care For these species additional markers will be necessary to achieve a 100  identification success We further illustrate how such reference databases can improve local reference databases for forensic entomologistsWe wish to thank Neal Haskell Benoit Vincent Zehner Bartok/Bartak Kubik Daniel Whitmore Birtele Cerretti Mei Lopresti Tenga Leonide Gatti Dal Cortivo Bystrofski AndreiRuiz J Hamon Jens Amendt JD Wells JA Cammack CJ Picard and P Boehme for providing part of the specimens We thank also the teams of DNA and Microtraces Analysis INCC especially Dr F Hubrecht Ir L Bourguignon Dr S Vanpoucke and Dr F Noel for their support during our work Kelly Meiklejohn for fruitful discussions and Thomas Pape for providing information on the breeding and feeding habits of the Sarcophaga species The comments of three anonymous referees improved the manuscript substantially

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