Application of six IrisPlex SNPs and comparison of

Authors: Libing Yun Yan Gu Haseena Rajeevan Kenneth K Kidd

Publish Date: 2014/01/07

Volume: 128, Issue: 3, Pages: 447-453

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Abstract

DNAbased prediction for externally visible characteristics such as eye color is already a useful tool in forensic criminal investigations The IrisPlex system consisting of six single nucleotide polymorphisms SNPs and a prediction model was developed based on individuals from several European populations Other recent studies have developed a different prediction model also based on European populations In this study we compared two prediction models using the data for the six IrisPlex SNPs genotyped on 905 individuals from 12 different Eurasian populations All SNPs showed significant differences in allele frequencies among three groups of populations European genetically intermediate Khanty Uygur and Yakut and East Asian The two prediction models the FROGkb calculation based on the formula of Walsh et al 2011 and the Snipper calculation from Ruiz et al 2013 gave identical predictions of brown eye color for the four East Asian populations with complete data but did not give concordant predictions for many individuals in the seven intermediate and European populations Inconsistencies were mainly conclusive prediction by one model but not the other Of the 714 individuals with complete 6locus genotypes the two models gave 22  inconsistent predictions Eliminating the 306 individuals in the Korean and three Chinese populations in which the predictions were always consistent for brown eye color the inconsistencies among the remaining 408 individuals were 387  We conclude that more attention should be paid to predictive uncertainty/error Implementation of both prediction models in future forensic casework is one immediate way to highlight uncertaintyThis work was supported by the Opening Project of Key Laboratory of Evidence Science China University of Political Science and Law Ministry of Education 2012KFKT06 The work at Yale was funded primarily by Grant 2010DNBXK225 to KKK awarded by the National Institute of Justice Office of Justice Programs US Department of Justice Points of view in this document are those of the authors and do not necessarily represent the official position or policies of the US Department of Justice We would also like to thank all the collaborators who helped to collect the samples Special thanks are due the hundreds of individuals who volunteered to give blood samples for studies of gene frequency variation

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