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Title of Journal: Int J Legal Med

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Abbravation: International Journal of Legal Medicine

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Springer Berlin Heidelberg

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DOI

10.1007/jhep06(2012)105

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ISSN

1437-1596

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Identification of human DNA in forensic evidence b

Authors: Khanistha Watthanapanpituck Wansika Kiatpathomchai Eric Chu Nathinee Panvisavas
Publish Date: 2014/05/15
Volume: 128, Issue: 6, Pages: 923-931
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Abstract

A DNA test based on loopmediated isothermal amplification LAMP and colorimetric gold nanoparticle AuNP hybridization probe to detect the presence of human DNA in forensic evidence was developed The LAMP primer set targeted eight regions of the human cytochrome b and its specificity was verified against the DNA of 11 animal species which included animals closely related to humans such as chimpanzee and orangutan By using the AuNP probe sequencespecific LAMP product could be detected and the test result could be visualized through the change in color The limit of detection was demonstrated with reproducibility to be as low as 718 fg of genomic DNA which is equivalent to approximately 100 plasmid DNA copies containing the cytochrome b DNA target region A simple DNA extraction method for the commonly found forensic biological samples was also devised to streamline the test process This LAMPAuNP human DNA test showed to be a robust specific and costeffective tool for the forensic identification of human specimens without requiring sophisticated laboratory instrumentsKhanistha Watthanapanpituck was supported by the Australian Federal Police AFP Forensic Science Scholarship 2011 This project was approved by Mahidol University Institutional Review Board MUIRB by an Expedited Review Process COA No MUIRB 2012 1370708

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