Authors: Kuang Youlin Zhang Jianwei Gou Xin Zhang Li Weng Xiaodong Liu Xiuheng Zhu Hengchen Chen Zhiyuan
Publish Date: 2012/10/16
Volume: 19, Issue: 2, Pages: 177-181
Abstract
It has been shown that human prostate cancer PCa cells induced apoptotic death of the most potent antigenpresenting cells dendritic cells DCs which are responsible for the induction of specific antitumor immune responses Here we investigated the function of 41BB on protecting DCs from prostate cancerinduced apoptosis with an agonistic mAb to 41BB RM1 cells and DCs were coincubated for 48 h and DC apoptosis was assessed by Annexin Vassay TNFα and IL12 production were assessed by enzymelinked immunosorbent assay ELISA and Bcl2 and BclxL on DCs were analyzed by Western blot We have shown that coincubation of RM1 cells with DCs is accompanied by an increased level of DCs apoptosis Triggering 41BB on DCs resulted in increased resistance of DCs to RM1 cellsinduced apoptosis which was owing to the upregulated expression of Bcl2 and BclxL and increased secretion of TNFαand IL12 These results demonstrate that triggering 41BB on DCs could increased resistance of DCs to PCainduced apoptosis
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