Tumor Grade versus Expression of InvasionRelated

Authors: József Virga László Bognár Tibor Hortobágyi Gábor Zahuczky Éva Csősz Gergő Kalló Judit Tóth Gábor Hutóczki Judit ReményiPuskár László Steiner Almos Klekner

Publish Date: 2017/02/04

Volume: 24, Issue: 1, Pages: 35-43

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Abstract

Peritumoral infiltration is characteristic of astrocytomas even in lowgrade tumors Tumor cells migrate to neighbouring tissue and cause recurrence The extracellular matrix ECM plays a role in tumor invasion expression levels of its components’ have been linked to tumor invasion This study determines the mRNA and protein expression of 20 invasionrelated ECM components by examining nontumor brain grade IIIIII astrocytoma and glioblastoma samples Expression levels were measured by QRTPCR and massspectroscopy The connection between the expression pattern and tumor grade is statistically analyzed During the analysis of data key molecules brevican cadherin12 fibronectin and integrinβ1 correlating the most with tumor grade were selected While the mRNA level of brevican ErbB2 fibronectin integrinβ1 and versican discriminates lowgrade from highgrade gliomas of proteins RHAMM integrinα1 and MMP2 seems important The expression pattern was found to be distinctive for tumor grade as statistical classifiers are capable of identifying an unknown sample’s grade using them Furthermore normal brain and glioma expression patterns along with lowgrade astrocytoma and glioblastoma samples differ the most Determining the invasionrelated molecules’ expression profile provides extra information regarding the tumor’s clinical behavior Additionally identifying molecules playing a key role in glioma invasion could uncover potential therapeutic targets in the futureThis study was supported by Hungarian Brain Research Program Grant No KTIA 13 NAPAV/3 II/7 and A Klekner by the János Bolyai Scholarship of the Hungarian Academy of Sciences Supported by the ÚNKP16 3I New National Excellence Program of the Ministry of Human Capacities

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