Biologic Evaluation of Diabetes and Local Recurren

Authors: Xuebin Yang Yongjun Liu Haresh Mani Jeffrey Olson Gary Clawson Carla Caruso Richard Bruggeman John M Varlotto Dani S Zander Negar Rassaei

Publish Date: 2016/07/13

Volume: 23, Issue: 1, Pages: 73-77

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Abstract

A recent multicenter study led by our institution demonstrated that local recurrence of nonsmall cell lung cancer NSCLC was significantly more frequent in patients with diabetes raising the possibility of different tumor biology in diabetics Epithelialtomesenchymal transition EMT plays a key role in local tumor recurrence and metastasis In the present study we investigated differences of tumor microenvironment between patients with and without diabetes by examining expression of EMT markers Seventynine NSCLC patients were selected from the cohort of our early multicenter study These patients were classified into 4 groups 39 with adenocarcinoma with n = 19 and without n = 20 diabetes and 40 with squamous cell carcinoma with n = 20 and without n = 20 diabetes Immunohistochemical expression of eight EMT markers was analyzed including transforming growth factorbeta TGFβ epidermal growth factor receptor EGFR insulinlike growth factor 1 receptor IGF1R vimentin Ecadherin Ncadherin HtrA1 and betacatenin Five markers Ecadherin HtrA1 TGFβ IGF1R and vimentin demonstrated significantly higher expression in diabetics than in nondiabetics in both histology types Ncadherin had higher expression in diabetics though the difference did not reach statistical significance EGFR showed a higher expression in diabetics in squamous cell carcinoma only Betacatenin was the only marker with no difference in expression between diabetics versus nondiabetics Our findings suggest that diabetes is associated with enhanced EMT in NSCLC which may contribute to growth and invasiveness of NSCLC

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