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Title of Journal: Virus Genes

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Abbravation: Virus Genes

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Kluwer Academic Publishers-Plenum Publishers

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DOI

10.1002/chin.201314074

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1572-994X

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Fulllength sequence analysis of four IBDV strains

Authors: Daniel Petkov Erich Linnemann Darrell R Kapczynski Holly S Sellers
Publish Date: 2007/06/01
Volume: 34, Issue: 3, Pages: 315-326
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Abstract

Characterization of field isolate 9109 Lukert Edgar cell cultureadapted CCA and Edgar chicken embryoadapted CEA serotype 1 IBDV strains using fulllength genomic sequences is reported IBDV genomic segments A and B were sequenced and the nucleotide and deduced amino acid aa sequences were compared with previously reported fulllength sequenced IBDV strains We found that the viral protein VPX and amino acid sequences between aa 202–451 and 210–473 of VP2 but not the entire VP2 protein are the best representatives of the entire IBDV genome The greatest variability was found in the VP2 and 5′ noncoding region of segment B among IBDV strains The deduced amino acid sequences of the VP1 protein varies in length among the strains analyzed The RNAdependent RNApolymerase motifs within VP1 and the VP5 protein were highly conserved among isolates Although within the VP2 processing site amino acid sequence of Lukert was similar to the classical while the Edgar CCA and CEA were more similar to the very virulent strains it was determined that these strains have sequence characteristics of the classical strains In addition close relatedness between Lukert Edgar CCA and CEA was observed Although phylogenetic analysis of the VP1 VP3 and VP4 proteins indicated that 9109 is a classical type virus this isolate shares unique amino acid changes with very virulent strains within the same proteins Phylogenetic analysis of the 3′ and 5′ noncoding regions of segment A revealed that 9109 is more similar to the very virulent strains compared to the classical strains In the VP2 protein several amino acids were conserved between variant E and 9109 strains Thus it appears that 9109 isolate has characteristics of classical very virulent and variant strains Our analysis indicates that although VPX amino acid comparison may be initially useful for molecular typing fulllength genomic sequence analysis is essential for thorough molecular characterization as partial sequences may not designate a particular strain as very virulent classical or variant

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