Authors: Chuan Wang Run Wu FaDi Li Lei Liu XiaoLi Zhang ChunLin Zhao XiaoLong Diao HongWei Guan
Publish Date: 2011/02/12
Volume: 42, Issue: 3, Pages: 457-462
Abstract
Determination of the transcription level of cellular prion protein PrPC is essential for understanding its role in organisms and revealing mechanism of susceptibility and resistance to scrapie However the expression of prion protein PrP mRNA in sheep has not been quantified in great detail in digestive tract which is important during scrapie spread through oral route Herein we report on measurement of sheep PrP mRNA using absolute quantitative realtime RTPCR Total RNA was isolated from five different regions of the central nervous system CNS four regions of lymphoid system eleven regions of digestive tract and two reproductive organ tissues of eight sheep of two different genotypes ARR/ARQ and ARH/ARQ and PrP mRNA was quantified by realtime RTPCR using molecular beacon The results showed that highest levels of PrP mRNA were expressed in thalamus and cerebrum P 001 of CNS examined followed by cerebellum spinal cord and brain stem In peripheral organs examined lymph tissue showed moderate level of PrP expression similar to that in digestive tract and reproduction organs PrP expression levels in the same tissue of different genotype sheep had significant variation Our study provided the first detail tissuespecific and genotypespecific data of PrP mRNA expression in sheep for further studies of pathogenesis of prion diseasesThis work was supported by Doctoral Program of Higher Education Fund No 20060733006 Agricultural Biotechnology Research and Application Program in Gansu Province GNSW2007004 and the State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences 2008–2009
Keywords: