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Title of Journal: Virus Genes

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Abbravation: Virus Genes

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Springer US

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DOI

10.1007/bf02788176

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1572-994X

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Genotyping of human bocavirus using a restriction

Authors: Vanessa Ditt Sergei Viazov Ramona Tillmann Verena Schildgen Oliver Schildgen
Publish Date: 2007/12/11
Volume: 36, Issue: 1, Pages: 67-69
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Abstract

Sequencing analysis of the isolates of a recently identified pathogen associated with respiratory infections human bocavirus HBoV allowed for identification of two virus genotypes of the virus In the current article a new method for a simple and fast differentiation of HBoV genotypes in clinical materials is described The test includes an amplification of a 309 bp fragment of VP1/VP2 gene of HBoV from nasopharyngeal aspirates with a subsequent incubation of a PCR mix with the BstAPI endonuclease Upon such a digestion the DNA fragment derived from the genotype I HBoV isolates forms two fragments of 150 and 159 bp while that obtained from genotype 2 isolates remains unrestricted The developed technique may be used in epidemiological studies of HBoV infection and analysis of the potential differences in biological characteristics of HboV genotypes

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