Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Virus Genes

Search In Journal Title:

Abbravation: Virus Genes

Search In Journal Abbravation:

Publisher

Springer US

Search In Publisher:

DOI

10.1002/poc.1170

Search In DOI:

ISSN

1572-994X

Search In ISSN:
Search In Title Of Papers:

Instability in vitro of a PCV2 infectious clone co

Authors: Dipongkor Saha Jan V Doorsselaere Hans J Nauwynck
Publish Date: 2011/11/25
Volume: 44, Issue: 2, Pages: 258-261
PDF Link

Abstract

Porcine circovirus 2 PCV2 is a small circular singlestranded DNA virus that causes postweaning multisystemic wasting syndrome in pigs We obtained an infectious clone derived from PCV2 strain 1147 size 1767 bp with a 60 bp insertion in the cap–rep intergenic region at the 3′ end of the rep In vitro propagation and sequencing of virus showed mixed sequence for the cap–rep intergenic region Cloning of this region of virus from the passages 2 and 20 showed deletions of various size of the 60 bp insertion Viruses from the passage 20 showed deletions of the 60 bp insertion and had extra +1 to +5 or less −1 or −2 bp in the intergenic region as compared to 1147 These findings suggest that insertion of a 60bp DNA sequence at the 3′ end of the rep is unstable in vitro This finding can have implications for the genetic engineering of PCV2

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Molecular characterization of the glycoprotein genes of H5N1 influenza A viruses isolated in Israel and the Gaza Strip during 2006 outbreaks
  2. ORF43 of maize rayado fino virus is dispensable for systemic infection of maize and transmission by leafhoppers
  3. Sequence and phylogenetic analysis identifies a putative novel gyrovirus 3 genotype in ferret feces
  4. Combination of intratypic and intertypic recombinant events in EV71: a novel evidence for the “triple-recombinant” strains of genotype A viruses in Mainland China from 2008 to 2010
  5. Expression patterns of prion protein gene in differential genotypes sheep: quantification using molecular beacon real-time RT-PCR
  6. Phylogenetic analysis of the VP1 gene of Enterovirus 71 in Guangzhou during the high occurrence period of 2008
  7. A novel finding for enterovirus virulence from the capsid protein VP1 of EV71 circulating in mainland China
  8. Epidemiological investigation of Middle East respiratory syndrome coronavirus in dromedary camel farms linked with human infection in Abu Dhabi Emirate, United Arab Emirates
  9. Comparative genomic sequence analysis of novel Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Kenya and three other previously sequenced Helicoverpa spp. NPVs
  10. Genetic comparisons between lentogenic Newcastle disease virus isolated from waterfowl and velogenic variants
  11. Pathogenicity and insect transmission of a begomovirus complex between tomato yellow leaf curl virus and Ageratum yellow vein betasatellite
  12. Determination of the full length sequence of a chicken astrovirus suggests a different replication mechanism
  13. Identification of a new newcastle disease virus isolate from Indonesia represents an ancestral lineage of class II genotype XIII
  14. Sequencing of the Tamus red mosaic virus genome: further evidence that it is a distinct species of the genus Potexvirus
  15. Evolution and adaptation of hemagglutinin gene of human H5N1 influenza virus
  16. Full-length hepatitis B virus sequences from naïve patients with fluctuation of viral load during ADV monotherapy
  17. Vaccine derived bi- and multi-recombinant Sabin strains
  18. Genomic deletions and mutations resulting in the loss of eight genes reduce the in vivo replication capacity of Meleagrid herpesvirus 1
  19. Molecular characterization and phylogenetic analysis of the complete genome of a hepatitis E virus from European swine
  20. Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal
  21. Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal
  22. Full-length sequence analysis of four IBDV strains with different pathogenicities
  23. Diversity and prevalence of Brazilian bipartite begomovirus species associated to tomatoes
  24. Mutations of two transmembrane cysteines of hemagglutinin (HA) from influenza A H3N2 virus affect HA thermal stability and fusion activity
  25. Genotyping of human bocavirus using a restriction length polymorphism
  26. Identification and RNA segment assignment of six structural proteins of Scylla serrata reovirus
  27. Genetic characterization of Amazonian bovine papillomavirus reveals the existence of four new putative types
  28. Isolation of Virus from a SARS Patient and Genome-wide Analysis of Genetic Mutations Related to Pathogenesis and Epidemiology from 47 SARS-CoV Isolates
  29. HN gene C-terminal extension of Newcastle disease virus is not the determinant of the enteric tropism
  30. Resistance gene analogs involved in tolerant cassava–geminivirus interaction that shows a recovery phenotype
Search Result: