Authors: Eleni Paximadi Ioannis Karakasiliotis Eugenia Bolanaki Aris Krikelis Panayotis Markoulatos
Publish Date: 2007/08/07
Volume: 35, Issue: 3, Pages: 541-548
Abstract
A retrospective analysis of five Sabin intertypic recombinant strains isolated from human feacal specimens during the time period 1978–1985 in Greece was performed by RTPCR Restriction Fragment Length Polymorphism RFLP and sequencing Of the studied strains three EPA EPB EPC were found to be birecombinant Sabin3/Sabin2/Sabin3 S3/S2/S3 one strain was characterized as a probable S3/S2 CAV18 or CAV21S2/S1 multirecombinant EDP11 and one was identified as a tripartite one S3/S2/S1 EDP12 Samples EPA EPB and EPC presented a common recombination junction in the 2C genomic region Moreover strains EPA and EPB shared also the second recombination site in the 3D genomic region whereas the second recombination of EPC was also determined in 3D but in a different nucleotide position Strains EDP11 and EDP12 presented both identical recombination motifs and recombination sites The first was detected in the 2C genomic region and the second in the 3D region Strain EDP11 presented an interesting feature since a sequence of 120 nucleotides seems to have derived from a member of human enteroviruses species C CAV18 or CAV21 This finding is of great importance considering that this strain EDP11 was isolated from an area and time period where no Coxsackie A virus or poliovirus epidemics occurred Our study underlines the role of specific positions and motifs of the poliovirus genomic sequences involved in recombination events and prompts that Coxsackie A viruses belonging to human enterovirus species C genetically closely related to PV are considered as the possible counterparts of the recombinationThe present work was co funded by the European Union 75 and the Greek Ministry of Education 25 under the framework of the Education and Initial Vocational Training Program “Pythagoras II” Research project “Molecular detection of Enteroviruses in clinical samples and the environment Implications for the public health Code 5221311”
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