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Title of Journal: Virus Genes

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Abbravation: Virus Genes

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Springer US

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DOI

10.1002/cite.330611107

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1572-994X

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Identification and RNA segment assignment of six s

Authors: Yangyang Yuan Dongyang Fan Zhao Zhang Jifang Yang Jingwen Liu Jigang Chen
Publish Date: 2016/03/29
Volume: 52, Issue: 4, Pages: 556-560
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Abstract

Scylla serrata reovirus SsRV is one of the most prevalent viral pathogens of the mud crab S serrata The virus represents an unassigned novel genus in the Reoviridae family and contains 12 doublestranded RNA genomic segments Previous analysis of virion proteins concluded that SsRV contains at least eight structural proteins ranging from 25 to 160 kDa Here tandem timeofflight mass spectrometry and Western blotting were used to reidentify the structural proteins The results indicate that proteins encoded by SsRV segments S1 S3 S6 S9 S11 and S12 are structural proteinsThis research was supported by the Natural Science Foundation of Zhejiang Province of China Grant No LY13C190003 the Applied Research Programs of public welfare technology of Zhejiang Province of China Grant No 2014C32057 the National Natural Science Foundation of China Grant No 30800856 the Zhejiang Provincial Top Key Discipline of Modern Microbiology and Application Grant No KF20140005 and the Innovative Research Team of Ningbo Grant No 2015C110018 to JG

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