Effect of cortisol on cell proliferation and the e

Authors: Kenshi Sakayama Naohiko Mashima Teruki Kidani Tatsuhiko Miyazaki Haruyasu Yamamoto Hiroshi Masuno

Publish Date: 2007/06/05

Volume: 61, Issue: 3, Pages: 471-479

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Abstract

The aim of this study is to investigate whether cortisol inhibited cell proliferation and the expressions of lipoprotein lipase LPL a key enzyme involved in the energy metabolism in tumor cells and vascular endothelial growth factor VEGF a potent angiogenic factor in the tumor in cultures of OST cells a human osteosarcoma cell lineOST cells were treated for 48 h with or without cortisol To examine the effect of cortisol on cell proliferation the expression of proliferating cell nuclear antigen PCNA was examined by Western blotting and the amount of 3Hthymidine incorporated into DNA during the last 30 min of the 48h treatment period was measured To examine the effect of cortisol on the expression of LPL the activity and mass of LPL were measured in the extract of acetone/ether powder of cells and the amount of 35Smethionine incorporated into LPL during the last 2 h of the 48h treatment period was measured by immunoprecipitation The expression of VEGF was examined by immunohistochemistry and Western blottingThe amount of 3Hthymidine incorporated into DNA and the level of PCNA were lower in the cortisoltreated cultures than in the untreated cultures thus indicating that cortisol inhibited the proliferation of OST cells The synthetic rate and activity of LPL were lower in the cortisoltreated cultures than in the untreated cultures but no difference in the specific activity of LPL between the two cultures was observed thus indicating that cortisol inhibited LPL synthesis thereby resulting in a decreased LPL activity The expression of VEGF was lower in the cortisoltreated cultures than in the untreated cultures

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