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Title of Journal: Pflugers Arch Eur J Physiol

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Abbravation: Pflügers Archiv - European Journal of Physiology

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Springer-Verlag

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1432-2013

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Kv1 and Kir2 potassium channels are expressed in r

Authors: Ian D Millar Shanshan Wang Peter D Brown Margery A Barrand Stephen B Hladky
Publish Date: 2007/11/20
Volume: 456, Issue: 2, Pages: 379-391
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Abstract

The endothelial cells of the brain microvasculature which constitute the blood–brain barrier secrete K+ into brain interstitial fluid K+ channels are predicted to have a central role to play in this process The aim of the following study was to characterise K+ channels in primary cultures of endothelial cells isolated from rat brain microvessels by wholecell patch clamp and realtime polymerase chain reaction In the 4 h after plating the rat brain endothelial cells expressed predominantly a depolarisationactivated delayedrectifying outward K+ conductance and a timeindependent inwardly rectifying K+ conductance prominent at hyperpolarising potentials The outward current was inhibited by 1 mM 4aminopyridine 4AP 10 nM margatoxin and 100 nM dendrotoxinK indicating the involvement of Kv1 channels The half maximal activation voltage and time constants of activation and inactivation of the 4APsensitive current were similar to Kv13 The inwardly rectifying conductance was inhibited by Ba2+ in a dose and voltagedependent fashion the kinetics of which resembled Kir2 channels Quantification of messenger ribonucleic acid transcripts revealed Kv13  12 = 14 = 15 = 16 and Kir21 = 2  23 In currentclamp experiments both 4AP and Ba2+ depolarised the membrane potential In conclusion rat brain endothelial cells express Kv1 and Kir2 K+ channels both of which participate in setting membrane potential and could mediate K+ secretion into the brain interstitial fluid

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