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Title of Journal: Pflugers Arch Eur J Physiol

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Abbravation: Pflügers Archiv - European Journal of Physiology

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Springer Berlin Heidelberg

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1432-2013

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Sensitisation of TRPV4 by PARSubscript2/Subscri

Authors: Silvia Sostegni Alexei Diakov Peter McIntyre Nigel Bunnett Christoph Korbmacher Silke Haerteis
Publish Date: 2014/06/08
Volume: 467, Issue: 4, Pages: 687-701
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Abstract

Proteolytic activation of proteaseactivated receptor 2 PAR2 may represent a major mechanism of regulating the transient receptor potential vanilloid 4 TRPV4 nonselective cation channel in pathophysiological conditions associated with protease activation eg during inflammation To provide electrophysiological evidence for PAR2mediated TRPV4 regulation we characterised the properties of human TRPV4 heterologously expressed in Xenopus laevis oocytes in the presence and absence of coexpressed human PAR2 In outsideout patches from TRPV4 expressing oocytes we detected singlechannel activity typical for TRPV4 with a singlechannel conductance of about 100 pS for outward and 55 pS for inward currents The synthetic TRPV4 activator GSK1016790A stimulated TRPV4 mainly by converting previously silent channels into active channels with an open probability of nearly one In oocytes coexpressing TRPV4 and PAR2 PAR2 activation by trypsin or by specific PAR2 agonist SLIGRLNH2 potentiated the GSK1016790Astimulated TRPV4 wholecell currents several fold indicative of channel sensitisation Preincubation of oocytes with the calcium chelator 12bis2aminophenoxyethaneNNN′N′tetraacetic acid BAPTAAM did not reduce the stimulatory effect of PAR2 activation on TRPV4 which indicates that the effect is independent of intracellular calcium signalling Neutrophil elastase a biased agonist of PAR2 that does not induce intracellular calcium signalling also caused a PAR2dependent sensitisation of TRPV4 The Rhokinase inhibitor Y27362 abolished elastasestimulated sensitisation of TRPV4 which indicates that Rhokinase signalling plays a critical role in PAR2mediated TRPV4 sensitisation by the biased agonist neutrophil elastase During acute inflammation neutrophil elastase may sensitise TRPV4 by a mechanism involving biased agonism of PAR2 and activation of RhokinaseThe expert technical assistance of Ralf Rinke is gratefully acknowledged This study was supported by a PhD fellowship from the Bayerische Forschungsstiftung SS and by NHMRC grants 63303 1031886 1046860 and 1049682 and Monash University NWB PM

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