Effect of heat and autoclave on allergenicity of p

Authors: KothBongWooRi Kim SoYoung Lee EuJin Song KyuEarn Kim DongHyun Ahn

Publish Date: 2011/04/30

Volume: 20, Issue: 2, Pages: 455-459

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Abstract

This study was performed to investigate the effects of heat and autoclave treatment on the binding ability of porcine serum albumin PSA to IgG and the serum of patients who were allergic to pork Heat treatment at 100°C for 20 min on PSA was able to decrease the binding ability of PSA to IgG to 52 The binding ability of PSA to IgG was also reduced to 29 by autoclave treatment of PSA at 121°C for 30 min The binding ability of PSA autoclaved for 30 min to serum also decreased to 10 SDSPAGE and immunoblotting of PSA heated at 100°C for 20 min revealed that the intensity of the PSA band decreased and its presence was barely recognized by IgG In the case of autoclave treatment for 5 min PSA was degraded to lowmolecularweight peptides and IgG and serum did not recognize them Therefore autoclave treatment can effectively reduce the allergenicity of PSA

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