Authors: Jinglong Liang Liqiong Guo Ping Sun Binghua Jiang Junfang Lin Weixiong Guo Hua Wan
Publish Date: 2016/06/30
Volume: 25, Issue: 3, Pages: 795-801
Abstract
Phenylpropanoids are widely used in food supplements pharmaceuticals and cosmetics with diverse benefits to human health Transcinnamic acid or pcoumaric acid is usually used as the starting precursor to produce phenylpropanoids Synthetic bioengineering of microbial cell factories offers a sustainable and flexible alternative method for obtaining these compounds In this study a constitutive expression system consisting of Rhodotorula glutinis phenylalanine/tyrosine ammonia lyase was developed to produce a phenylpropanoic acid precursor in Escherichia coli To improve transcinnamic acid and pcoumaric acid production BioBrick optimization was investigated causing a 72 and 142fold increase in the yield of these compounds respectively The optimum strain was capable of de novo producing 7881 mg/L of transcinnamic acid and 3467 mg/L of pcoumaric acid in a shake flask culture The work presented here paves the way for the development of a sustainable and economical process for microbial production of a phenylpropanoic acid precursor
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