Authors: Yongqing Zhang Qingping Wu Jumei Zhang Xiuhua Yang
Publish Date: 2015/06/30
Volume: 24, Issue: 3, Pages: 987-993
Abstract
The ozone impact on Pseudomonas aeruginosa ATCC27853 cells was studied Ozoneinduced loss of K+ Mg2+ and ATP was measured using inductively coupled plasma/mass spectrometry and a bioluminescence assay Maximum releases of K+ and Mg2+ were achieved at 037 mg/L of ozone after 2 min with a killing rate of culturable bacteria greater than 93 Maximum release of ATP was attained at 042 mg/L of ozone after 3 min Transmission electron microscopy showed that shapes of treated cells were integrated but cytoplasmic agglutinations and vacuoles appeared Ozone treatment caused lysis of P aeruginosa cells to be sensitive to sodium dodecyl sulfate insensitive to NaOH and subject to inactivation by proteinase K A combined action of cytoplasm agglutination protein denaturation and membrane permeabilization rather than cell lysis leads to nonculturability of P aeruginosa cells
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