Realtime tracking of adipose tissuederived stem

Authors: Junjie Yang Zhiqiang Liu Jinming Zhang Haibin Wang Shunyin Hu Jianfeng Liu Changyong Wang Yundai Chen

Publish Date: 2012/09/01

Volume: 28, Issue: 3, Pages: 385-396

PDF Link

Abstract

Adipose tissuederived stem cells ADSCs has shown promise in the emerging field of regenerative medicine Many studies have highlighted the importance of coadministering a “scaffold” for increasing intramyocardial retention of stem cells In this work an optimized method was developed for efficient transduction of ADSCs with a lentiviral vector carrying a triplefusion reporter gene that consists of firefly luciferase monomeric red fluorescence protein and truncated thymidine kinase fluc–mrfp–ttk The transduced ADSCs were assessed on biological performance and transplanted into infarcted heart with fibrin scaffolds In vivo cell retention was tracked by bioluminescence imaging BLI and micro positron emission tomography/computed tomography PET/CT imaging Histological assessment was performed for regeneration potentials The results showed that lentiviral transduction did not influence cell functions In vitro imaging analysis showed a robust linear correlation between cell numbers and BLI signals R 2 = 099 as well as between cell numbers and radiotracer uptakes R 2 = 098 Transduced ADSCs were visualized in the heart under both BLI and PET/CT imaging contributing to cardiomyocyte regeneration and angiogenesis in the implanted areas Compared with BLI monitoring PET/CT data provided precise localization for cell retention Thus a combination of imaging modalities can assist in reliable and efficient monitoring of transplanted cells holding great potential for the transplantation of injectable scaffolds encapsulating stem cells in treating heart diseaseThis work was supported by grants from National High Technology Research and Development Program of China No 2011AA020101 and No 2012AA020506 Key Program of National Natural Science Foundation of China No 31030032 and National Natural Science Funds for Distinguished Young Scholar No 31125013 There are no relationships with industry The authors thank Professor Sanjiv S Gambhir Stanford University Radiology Department for kindly providing DNA vectors for production of lentivirus carrying mrfp–flu–ttk fusion reporter gene the authors also thank Wu Liangliang BSc and Feng Lanlan BSc for technical assistance in cell sorting histological preparation and staining

Keywords: