Journal Title
Title of Journal: Eur Biophys J
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Abbravation: European Biophysics Journal
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Publisher
Springer-Verlag
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Authors: Victoria Vitkova Julia Genova Isak Bivas
Publish Date: 2004/05/26
Volume: 33, Issue: 8, Pages: 706-714
Abstract
The passive water permeability of a lipid vesicle membrane was studied related to the hydrostatic not osmotic pressure difference between the inner and the outer side of the vesicle in a water environment without additives Each pressure difference was created by sucking a vesicle into a micropipette at a given sucking pressure The part of the membrane sucked into the micropipette the projection length was measured as a function of time The time dependence can be divided into two intervals We put forward the idea that smoothing of membrane defects accompanied by an increase of the membrane area takes place during the initial time interval which results in a faster increase of the projection length In the second time interval the volume of the vesicle decreases due to the permeability of its membrane and the increase of the projection length is slower The hidden area and the water permeability of a typical lipid bilayer were estimated The measured permeability conjugated to the hydrostatic pressure difference is an order of magnitude higher than the known value of the permeability conjugated to the osmotic pressure difference A hypothesis based on pore formation is proposed as an explanation of this experimental resultThe authors wish to express their gratitude to Dr MD Mitov for fruitful discussions This work was carried out in the FrenchBulgarian Laboratory “Vesicles and Membranes” supported by CNRS France and the Bulgarian Academy of Sciences and Sofia University Bulgaria The contribution of the Bulgarian National Science Foundation contract F823 is acknowledgedLet j0 be the molar flow of solvent molecules and j1 be the molar flow of solute molecules through the membrane The volume flow J 0textv through the membrane the volume flow of both the solvent and the solute is J 0textv = j 0 v 0 + j 1 v 1 The permeability DΔP can be expressed from Eqs 5 B8 B16 B17 and B18 as a function of Rp Rv dL/dt and dS/dt Taking into account that phi left R textp mathordleft/ vphantom R textp R textv right kernnulldelimiterspace R textv right approx 2 see Eq B7 this approximation has been used by Olbrich et al 2000 and replacing sqrt 1 left R textp right2 mathordleft/ vphantom left R textp right2 left R textv right2 right kernnulldelimiterspace left R textv right2 with left 1 left R textp right2 mathordleft/ vphantom left R textp right2 2left R textv right2 right kernnulldelimiterspace 2left R textv right2 right we obtain Eq 9 The error due to these two approximations is of the order of 1 mathordleft/ vphantom 1 6 right kernnulldelimiterspace 6 times left R textp right2 mathordleft/ vphantom left R textp right2 left R textv right2 right kernnulldelimiterspace left R textv right2 approx 002 It is negligible in comparison to the experimental errors of the measurements
Keywords:
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