Realtime and noninvasive monitoring of respiratio

Authors: Toshiya Sakata Izumi Makino Sayaka Kita

Publish Date: 2011/02/27

Volume: 40, Issue: 5, Pages: 699-704

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Abstract

In this report we propose a novel evaluation method of embryo activity describing the realtime and noninvasive electrical monitoring of embryo activity caused by fertilization of the sea urchin using a biologicallycoupled fieldeffect transistor bioFET comprised of semiconductorbased biosensing devices The detection principle of bioFET is based on the potentiometric detection of charge density change at the gate insulator which includes changes of hydrogen ion concentration corresponding to pH variation The surface potential at the gate surface of the bioFET increased after the introduction of sperms into the ova resulting in fertilization on the gate sensing area The positive shift of surface potential indicates the increase of positive charges of hydrogen ions generated by dissolved carbon dioxide in artificial sea water based on respiration activity of the embryo Moreover the electrical signal of embryo activity is suppressed due to the inhibition of cytokinesis by introduction of cytochalasin B The platform based on the bioFET is expected to be a realtime labelfree and noninvasive detection system not only in fundamental studies of embryo activity but also in the evaluation of embryo quality for in vitro fertilizationThe authors wish to thank Prof K Kataoka Prof K Ishihara and Dr A Matsumoto of the University of Tokyo in Japan Dr Y Miyahara and Dr T Tateishi of National Institute for Materials Science in Japan Mr Y Nakajima of Ryokusei MES Co Ltd in Japan and Dr M Kamahori of Hitachi Ltd in Japan for their help and useful discussion This work was partly supported by THE MURATA SCIENCE FOUNDATION Japan

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