Journal Title
Title of Journal: Eur Biophys J
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Abbravation: European Biophysics Journal
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Publisher
Springer International Publishing
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Authors: Patricia S Kumagai Ricardo DeMarco Jose L S Lopes
Publish Date: 2017/03/03
Volume: 46, Issue: 7, Pages: 599-606
Abstract
The unordered secondary structural content of an intrinsically disordered protein IDP is susceptible to conformational changes induced by many different external factors such as the presence of organic solvents removal of water changes in temperature binding to partner molecules and interaction with lipids and/or other ligands In order to characterize the highflexibility nature of an IDP circular dichroism CD spectroscopy is a particularly useful method due to its capability of monitoring both subtle and remarkable changes in different environments relative ease in obtaining measurements the small amount of sample required and the capability for sample recovery sample not damaged and others Using synchrotron radiation as the light source for CD spectroscopy represents the stateoftheart version of this technique with feasibility of accessing the lower wavelength UV region and therefore presenting a series of advantages over conventional circular dichroism cCD to monitor a protein conformational behavior check protein stability detect ligand binding and many others In this paper we have performed a comparative study using cCD and SRCD methods for investigating the secondary structure and the conformational behavior of natively unfolded proteins MEG14 and soybean trypsin inhibitor We show that the SRCD technique greatly improves the analysis and accuracy of the studies on the conformations of IDPsThe authors thank Prof BA Wallace for helpful discussions This work was supported by a postdoctoral research fellowship Grant Number 150417/20160 to PSK and 407337/20130 from CNPqBrazil and paired UK/Brazil partnering grants from Sao Paulo Research Foundation FAPESP to JLSL and the UK Biotechnology and Biological Sciences Research Council BBSRC Grant Number 15/503472 The authors are grateful for the access to the AUCD beamline on ASTRID2 at University of Aarhus Denmark beamtime grants to JLSL and PSK
Keywords:
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