Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Eur Biophys J

Search In Journal Title:

Abbravation: European Biophysics Journal

Search In Journal Abbravation:

Publisher

Springer-Verlag

Search In Publisher:

DOI

10.1002/jlac.199019900138

Search In DOI:

ISSN

1432-1017

Search In ISSN:
Search In Title Of Papers:

Human erythrocyte flickering temperature ATP con

Authors: David Szekely Tsz Wai Yau Philip W Kuchel
Publish Date: 2009/05/31
Volume: 38, Issue: 7, Pages: 923-939
PDF Link

Abstract

Images of human erythrocytes from a healthy donor were recorded under differential interference contrast DIC microscopy they were acquired rapidly ~336 Hz and the intensity of the centermost pixel of each cell was recorded for ~60 s 20000 values Various techniques were used to analyze the data including detrended fluctuation analysis DFA and multiscale entropy MSE however power spectrum analysis was deemed the most appropriate for metrifying and comparing results This analysis was used to compare cells from young and old populations and after perturbing normal conditions with changes in temperature adenosine triphosphate ATP concentration using NaF an inhibitor of glycolysis and αtoxin a poreforming molecule used to permeabilize red cells to ATP and water transport rates using glycerol and pchloromercuriphenylsulfonic acid pCMBS to inhibit aquaporins AQPs There were measurable differences in the membrane fluctuation characteristics in populations of young and old cells but there was no significant change in the flickering time series on changing the temperature of an individual cell by depleting it of ATP or by competing with the minor water exchange pathway via AQP3 using glycerol However pCMBS which inhibits AQP1 the major water exchange pathway inhibited flickering in all cells and yet it was restored by the membrane intercalating species dibutyl phthalate DBP We developed a computer model to simulate acquired displacement spectral time courses and to evaluate various methods of data analysis and showed how the flexibility of the membrane as defined in the model affects the flickering time course


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:

  1. Interrelation between HeLa-S3 cell transfection and hemolysis in red blood cell suspension using pulsed ultrasound of various duty cycles
  2. Inter-subunit disulfide cross-linking in homomeric and heteromeric P2X receptors
  3. Lambda exonuclease digestion of CGG trinucleotide repeats
  4. A molecular dynamics study of the bee venom melittin in aqueous solution, in methanol, and inserted in a phospholipid bilayer
  5. Liposome complexation efficiency monitored by FRET: effect of charge ratio, helper lipid and plasmid size
  6. Effect of headgroup on the dipole potential of phospholipid vesicles
  7. Ca 2+ signaling in striated muscle: the elusive roles of triadin, junctin, and calsequestrin
  8. Anticooperativity in diffusion-controlled reactions with pairs of anisotropic domains: a model for the antigen–antibody encounter
  9. A method for simultaneously delineating multiple targets in 3D-FISH using limited channels, lasers, and fluorochromes
  10. Deformation of intracellular endosomes under a magnetic field
  11. Another look at the interaction between mitochondrial cytochrome c and flavocytochrome b 2
  12. Molecular dynamics simulations and membrane protein structure quality
  13. Real-time and noninvasive monitoring of respiration activity of fertilized ova using semiconductor-based biosensing devices
  14. PKD2L1/PKD1L3 channel complex with an alkali-activated mechanism and calcium-dependent inactivation
  15. Dynamics of C-phycocyanin in various deuterated trehalose/water environments measured by quasielastic and elastic neutron scattering
  16. Ultrasound enhanced methanol penetration of zebrafish ( Danio rerio ) embryos measured by permittivity changes using impedance spectroscopy
  17. Microinjection in combination with microfluorimetry to study proton diffusion along phospholipid membranes
  18. Preferential DNA photocleavage potency of Zn(II) over Ni(II) derivatives of carboxymethyl tetracationic porphyrin: the role of the mode of binding to DNA
  19. Modeling the hydration of proteins: prediction of structural and hydrodynamic parameters from X-ray diffraction and scattering data
  20. Kinetics of phospholipid insertion into monolayers containing the lung surfactant proteins SP-B or SP-C
  21. Advantages of synchrotron radiation circular dichroism spectroscopy to study intrinsically disordered proteins
  22. Permeability and the hidden area of lipid bilayers
  23. Viral ion channel proteins in model membranes: a comparative study by X-ray reflectivity
  24. Blood oxygenation using microbubble suspensions
  25. Relaxivities of paramagnetic liposomes: on the importance of the chain type and the length of the amphiphilic complex
  26. Novel methods for preparing phospholipid coated microbubbles
  27. From kinetics to imaging: an NMR odyssey—a festschrift symposium in honour of Philip William Kuchel
  28. Soft perforation of cardiolipin-containing planar lipid bilayer membrane by cytochrome c and H 2 O 2
  29. Spectral broadening of the Soret band in myoglobin: an interpretation by the full spectrum of low-frequency modes from a normal modes analysis
  30. Intermittent contact mode AFM investigation of native plasma membrane of Xenopus laevis oocyte
  31. Neutron spin echo studies on ferritin: free-particle diffusion and interacting solutions

Search Result: