Journal Title
Title of Journal: Eur Biophys J
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Abbravation: European Biophysics Journal
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Publisher
Springer-Verlag
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Authors: Florence Lederer
Publish Date: 2011/04/19
Volume: 40, Issue: 12, Pages: 1283-1299
Abstract
Yeast flavocytochrome b 2 tranfers reducing equivalents from lactate to oxygen via cytochrome c and cytochrome c oxidase The enzyme catalytic cycle includes FMN reduction by lactate and reoxidation by intramolecular electron transfer to heme b 2 Each subunit of the soluble tetrameric enzyme consists of an N terminal b 5like hemebinding domain and a C terminal flavodehydrogenase In the crystal structure FMN and heme are face to face and appear to be in a suitable orientation and at a suitable distance for exchanging electrons But in one subunit out of two the heme domain is disordered and invisible This raises a central question is this mobility required for interaction with the physiological acceptor cytochrome c which only receives electrons from the heme and not from the FMN The present review summarizes the results of the variety of methods used over the years that shed light on the interactions between the flavin and heme domains and between the enzyme and cytochrome c The conclusion is that one should consider the interaction between the flavin and heme domains as a transient one and that the cytochrome c and the flavin domain docking areas on the heme b 2 domain must overlap at least in part The heme domain mobility is an essential component of the flavocytochrome b 2 functioning In this respect the enzyme bears similarity to a variety of redox enzyme systems in particular those in which a cytochrome b 5like domain is fused to proteins carrying other redox functions
Keywords:
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- Inter-subunit disulfide cross-linking in homomeric and heteromeric P2X receptors
- Lambda exonuclease digestion of CGG trinucleotide repeats
- A molecular dynamics study of the bee venom melittin in aqueous solution, in methanol, and inserted in a phospholipid bilayer
- Liposome complexation efficiency monitored by FRET: effect of charge ratio, helper lipid and plasmid size
- Effect of headgroup on the dipole potential of phospholipid vesicles
- Ca 2+ signaling in striated muscle: the elusive roles of triadin, junctin, and calsequestrin
- Anticooperativity in diffusion-controlled reactions with pairs of anisotropic domains: a model for the antigen–antibody encounter
- A method for simultaneously delineating multiple targets in 3D-FISH using limited channels, lasers, and fluorochromes
- Deformation of intracellular endosomes under a magnetic field
- Molecular dynamics simulations and membrane protein structure quality
- Real-time and noninvasive monitoring of respiration activity of fertilized ova using semiconductor-based biosensing devices
- PKD2L1/PKD1L3 channel complex with an alkali-activated mechanism and calcium-dependent inactivation
- Dynamics of C-phycocyanin in various deuterated trehalose/water environments measured by quasielastic and elastic neutron scattering
- Ultrasound enhanced methanol penetration of zebrafish ( Danio rerio ) embryos measured by permittivity changes using impedance spectroscopy
- Microinjection in combination with microfluorimetry to study proton diffusion along phospholipid membranes
- Preferential DNA photocleavage potency of Zn(II) over Ni(II) derivatives of carboxymethyl tetracationic porphyrin: the role of the mode of binding to DNA
- Modeling the hydration of proteins: prediction of structural and hydrodynamic parameters from X-ray diffraction and scattering data
- Kinetics of phospholipid insertion into monolayers containing the lung surfactant proteins SP-B or SP-C
- Advantages of synchrotron radiation circular dichroism spectroscopy to study intrinsically disordered proteins
- Permeability and the hidden area of lipid bilayers
- Viral ion channel proteins in model membranes: a comparative study by X-ray reflectivity
- Blood oxygenation using microbubble suspensions
- Human erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulation
- Relaxivities of paramagnetic liposomes: on the importance of the chain type and the length of the amphiphilic complex
- Novel methods for preparing phospholipid coated microbubbles
- From kinetics to imaging: an NMR odyssey—a festschrift symposium in honour of Philip William Kuchel
- Soft perforation of cardiolipin-containing planar lipid bilayer membrane by cytochrome c and H 2 O 2
- Spectral broadening of the Soret band in myoglobin: an interpretation by the full spectrum of low-frequency modes from a normal modes analysis
- Intermittent contact mode AFM investigation of native plasma membrane of Xenopus laevis oocyte
- Neutron spin echo studies on ferritin: free-particle diffusion and interacting solutions
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