Journal Title
Title of Journal: Eur Biophys J
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Abbravation: European Biophysics Journal
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Publisher
Springer-Verlag
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Authors: Robert Y Wang Mo Guan David M Rawson Tiantian Zhang
Publish Date: 2007/11/06
Volume: 37, Issue: 6, Pages: 1039-1044
Abstract
Studies on permittivity changes in fish embryos measured by impedance spectroscopy after ultrasound treatment during exposure to cryoprotectant is reported here for the first time The permittivity changes of zebrafish embryos in cryoprotectant solutions before and after ultrasound treatment were measured using impedance spectroscopy Zebrafish Danio rerio embryos at 50 epiboly stage were exposed to 2 M methanol for 25 min before ultrasound treatment for 5 min at 22°C Embryos were treated with ultrasound in different frequencies 24 and 48 kHz and voltages 50 100 150 and 175 V combinations The results showed a clear increasing trend of permittivity from voltage 50 to 175 V over lower impedance frequency range of 10–103 Hz indicating increased methanol penetration into the embryos after ultrasound treatment The embryo survival was not compromised after ultrasound treatment under conditions used in the present study The use of impedance spectroscopy technique provides a useful noneinvasive tool for detecting changes of cryoprotectant penetration in fish embryos after ultrasound treatment The technique is especially useful for the selection of the suitable cryoprotectants in embryo cryopreservation and may also allow quantitative measurements in embryo membrane permeability studies
Keywords:
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Other Papers In This Journal:
- Interrelation between HeLa-S3 cell transfection and hemolysis in red blood cell suspension using pulsed ultrasound of various duty cycles
- Inter-subunit disulfide cross-linking in homomeric and heteromeric P2X receptors
- Lambda exonuclease digestion of CGG trinucleotide repeats
- A molecular dynamics study of the bee venom melittin in aqueous solution, in methanol, and inserted in a phospholipid bilayer
- Liposome complexation efficiency monitored by FRET: effect of charge ratio, helper lipid and plasmid size
- Effect of headgroup on the dipole potential of phospholipid vesicles
- Ca 2+ signaling in striated muscle: the elusive roles of triadin, junctin, and calsequestrin
- Anticooperativity in diffusion-controlled reactions with pairs of anisotropic domains: a model for the antigen–antibody encounter
- A method for simultaneously delineating multiple targets in 3D-FISH using limited channels, lasers, and fluorochromes
- Deformation of intracellular endosomes under a magnetic field
- Another look at the interaction between mitochondrial cytochrome c and flavocytochrome b 2
- Molecular dynamics simulations and membrane protein structure quality
- Real-time and noninvasive monitoring of respiration activity of fertilized ova using semiconductor-based biosensing devices
- PKD2L1/PKD1L3 channel complex with an alkali-activated mechanism and calcium-dependent inactivation
- Dynamics of C-phycocyanin in various deuterated trehalose/water environments measured by quasielastic and elastic neutron scattering
- Microinjection in combination with microfluorimetry to study proton diffusion along phospholipid membranes
- Preferential DNA photocleavage potency of Zn(II) over Ni(II) derivatives of carboxymethyl tetracationic porphyrin: the role of the mode of binding to DNA
- Modeling the hydration of proteins: prediction of structural and hydrodynamic parameters from X-ray diffraction and scattering data
- Kinetics of phospholipid insertion into monolayers containing the lung surfactant proteins SP-B or SP-C
- Advantages of synchrotron radiation circular dichroism spectroscopy to study intrinsically disordered proteins
- Permeability and the hidden area of lipid bilayers
- Viral ion channel proteins in model membranes: a comparative study by X-ray reflectivity
- Blood oxygenation using microbubble suspensions
- Human erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulation
- Relaxivities of paramagnetic liposomes: on the importance of the chain type and the length of the amphiphilic complex
- Novel methods for preparing phospholipid coated microbubbles
- From kinetics to imaging: an NMR odyssey—a festschrift symposium in honour of Philip William Kuchel
- Soft perforation of cardiolipin-containing planar lipid bilayer membrane by cytochrome c and H 2 O 2
- Spectral broadening of the Soret band in myoglobin: an interpretation by the full spectrum of low-frequency modes from a normal modes analysis
- Intermittent contact mode AFM investigation of native plasma membrane of Xenopus laevis oocyte
- Neutron spin echo studies on ferritin: free-particle diffusion and interacting solutions
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