Journal Title
Title of Journal: Eur Biophys J
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Abbravation: European Biophysics Journal
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Publisher
Springer-Verlag
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Authors: Thomas StarkePeterkovic Ronald J Clarke
Publish Date: 2009/01/09
Volume: 39, Issue: 1, Pages: 103-
Abstract
The dipole potentials ψ d of phospholipid vesicles composed of pure dimyristoylphosphatidylcholine DMPC or vesicles in which 50 mol of the DMPC was substituted by dimyristoylphosphatidylserine DMPS dimyristoylphosphatidylglycerol DMPG dimyristoylethanolamine DMPE dimyristoylphosphatidic acid DMPA or monomyristoylphosphatidylcholine MMPC were measured via a fluorescent ratiometric method utilizing the probe di8ANEPPS The PS and PG headgroups were found to cause only minor changes in ψ d PE caused an increase in ψ d of 51 mV This could be explained by a decrease in the dielectric constant of the glycerol backbone region as well as a movement of the P−–N+ dipole of the less bulky PE headgroup to a position more parallel to the membrane surface than in PC The negatively charged PA headgroup increases ψ d by 215 mV relative to PC alone This indicates that the positive pole of the dipole predominantly responsible for the dipole potential is located at a position closer to the interior of the membrane than the phosphate group The increase in the charge of the negative pole of the dipole by the phosphate group of PA increases the electrical potential drop across the lipid headgroup region The incorporation of the single chain lipid MMPC into the membrane causes a decrease in ψ d of 142 mV This can be explained by a decrease in packing density within the membrane of carbonyl dipoles from the sn2 chain of DMPC The results presented should contribute to a better understanding of the electrical effect of lipid headgroups on the functioning of membrane proteinsR J Clarke acknowledges with gratitude financial support from the Australian Research Council Discovery Grant DP0208282 and from the Australian Research Council/National Health and Medical Research Council funded Research Network “Fluorescence Applications in Biotechnology and the Life Sciences” RN0460002
Keywords:
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