Journal Title
Title of Journal: Eur Biophys J
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Abbravation: European Biophysics Journal
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Publisher
Springer-Verlag
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Authors: Wolfgang Häussler
Publish Date: 2008/02/13
Volume: 37, Issue: 5, Pages: 563-571
Abstract
The dynamics of proteins are often studied by means of quasielastic neutron scattering QENS for example by timeofflight methods The spatial dimensions 10–20 nm present in protein solutions are accessible by neutron scattering In this article a systematic study of diffusive dynamics of ferritin and apoferritin =ferritin without iron core is presented Apoferritin consists of a spherical shell built of 24 protein units and carries net negative charge at pH 5 We have studied diffusive dynamics of ferritin solutions by neutron spin echo NSE We pay attention to an important feature of this technique compared to other QENS methods which being the usage of a broad wavelength band Using a more sophisticated fit function than usually used in NSE we find as expected in low concentrated systems that the diffusion coefficient approaches the freeparticle value of apoferritin and coincides with the diameter of the apoferritin shell 122 nm In interacting solutions the NSE results reveal that the dynamic picture of this complex liquid is dominated by slowing down of the dynamics In lowsalt solutions a structure factor peak appears due to ordering of the ferritin molecules on the length scale of several intermolecular distances We discuss the usage of different NSE fit functions for interacting solutions near the structure factor peak Comparison of the dependence of elastic and dynamic data on the scattering vector value shows the influence of indirect interactions on the dynamic picture irrespective of the way of data analysis which being necessary due to the broad wavelength spectrumAdvanced neutron scattering and complementary techniques to study biological systems Contributions from the meetings “Neutrons in Biology” STFC Rutherford Appleton Laboratory Didcot UK 11–13 July and “Proteins At Work 2007” Perugia Italy 28–30 May 2007
Keywords:
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- Interrelation between HeLa-S3 cell transfection and hemolysis in red blood cell suspension using pulsed ultrasound of various duty cycles
- Inter-subunit disulfide cross-linking in homomeric and heteromeric P2X receptors
- Lambda exonuclease digestion of CGG trinucleotide repeats
- A molecular dynamics study of the bee venom melittin in aqueous solution, in methanol, and inserted in a phospholipid bilayer
- Liposome complexation efficiency monitored by FRET: effect of charge ratio, helper lipid and plasmid size
- Effect of headgroup on the dipole potential of phospholipid vesicles
- Ca 2+ signaling in striated muscle: the elusive roles of triadin, junctin, and calsequestrin
- Anticooperativity in diffusion-controlled reactions with pairs of anisotropic domains: a model for the antigen–antibody encounter
- A method for simultaneously delineating multiple targets in 3D-FISH using limited channels, lasers, and fluorochromes
- Deformation of intracellular endosomes under a magnetic field
- Another look at the interaction between mitochondrial cytochrome c and flavocytochrome b 2
- Molecular dynamics simulations and membrane protein structure quality
- Real-time and noninvasive monitoring of respiration activity of fertilized ova using semiconductor-based biosensing devices
- PKD2L1/PKD1L3 channel complex with an alkali-activated mechanism and calcium-dependent inactivation
- Dynamics of C-phycocyanin in various deuterated trehalose/water environments measured by quasielastic and elastic neutron scattering
- Ultrasound enhanced methanol penetration of zebrafish ( Danio rerio ) embryos measured by permittivity changes using impedance spectroscopy
- Microinjection in combination with microfluorimetry to study proton diffusion along phospholipid membranes
- Preferential DNA photocleavage potency of Zn(II) over Ni(II) derivatives of carboxymethyl tetracationic porphyrin: the role of the mode of binding to DNA
- Modeling the hydration of proteins: prediction of structural and hydrodynamic parameters from X-ray diffraction and scattering data
- Kinetics of phospholipid insertion into monolayers containing the lung surfactant proteins SP-B or SP-C
- Advantages of synchrotron radiation circular dichroism spectroscopy to study intrinsically disordered proteins
- Permeability and the hidden area of lipid bilayers
- Viral ion channel proteins in model membranes: a comparative study by X-ray reflectivity
- Blood oxygenation using microbubble suspensions
- Human erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulation
- Relaxivities of paramagnetic liposomes: on the importance of the chain type and the length of the amphiphilic complex
- Novel methods for preparing phospholipid coated microbubbles
- From kinetics to imaging: an NMR odyssey—a festschrift symposium in honour of Philip William Kuchel
- Soft perforation of cardiolipin-containing planar lipid bilayer membrane by cytochrome c and H 2 O 2
- Spectral broadening of the Soret band in myoglobin: an interpretation by the full spectrum of low-frequency modes from a normal modes analysis
- Intermittent contact mode AFM investigation of native plasma membrane of Xenopus laevis oocyte
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