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Title of Journal: Cytotechnology

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Abbravation: Cytotechnology

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Springer Netherlands

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DOI

10.1007/bf00584859

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ISSN

1573-0778

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Current status of transcriptional regulation syste

Authors: Tobias May Hansjörg Hauser Dagmar Wirth
Publish Date: 2006/05/20
Volume: 50, Issue: 1-3, Pages: 109-
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Abstract

Many attempts have been undertaken to control transgene activity in mammalian cells This is of importance for both applied biotechnology and basic research activities State of the art regulatory systems use elements for transgene regulation which are unrelated to host regulatory networks and thus do not interfere with endogenous activities Most of these regulation systems consist of transregulators and transregulator responding promoter elements that are derived from non mammalian origin Apart from the tetracycline Tet regulated system which is most widely used for conditional gene expression at the moment a number of new systems were created These systems have been significantly refined and their performance makes them suitable for regulating transgenes not only in cellular systems but also in transgenic animals and for human therapeutic use


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  2. Efficiency of Plasmocin™ on various mammalian cell lines infected by mollicutes in comparison with commonly used antibiotics in cell culture: a local experience
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  4. Study of kinetic parameters for the production of recombinant rabies virus glycoprotein
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  6. Isolation and characterization of cancer stem cells from cervical cancer HeLa cells
  7. Flavonoids from Heliotropium subulatum exudate and their evaluation for antioxidant, antineoplastic and cytotoxic activities II
  8. Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions
  9. Immortalization of pig fibroblast cells by transposon-mediated ectopic expression of porcine telomerase reverse transcriptase
  10. Lithium chloride promotes neuronal differentiation of rat neural stem cells and enhances neural regeneration in Parkinson’s disease model
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  12. Cytotoxic responses of carnosic acid and doxorubicin on breast cancer cells in butterfly-shaped microchips in comparison to 2D and 3D culture
  13. Optimization and evaluation of electroporation delivery of siRNA in the human leukemic CEM cell line
  14. Implication of SUMO E3 ligases in nucleotide excision repair
  15. Induction of apoptosis with mitochondrial membrane depolarization by a glycyrrhetinic acid derivative in human leukemia K562 cells
  16. A fully autologous co-culture system utilising non-irradiated autologous fibroblasts to support the expansion of human keratinocytes for clinical use
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  18. Optimization of the isolation and cultivation of Cyprinus carpio primary hepatocytes
  19. A simple adhesion assay for studying interactions between platelets and endothelial cells in vitro
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