Authors: Bharat Singh Pooran M Sahu Ram A Sharma
Publish Date: 2016/11/30
Volume: 69, Issue: 1, Pages: 103-115
Abstract
The flavonoids are the largest group of phenolic compounds isolated from a wide range of higher plants These compounds work as antimicrobials antiinsect agents and protect plants from other types of biotic and abiotic stresses Various researchers have suggested that flavonoids possessed antioxidant antineoplastic and cytotoxic activities The main objective of this study was to test dichloromethane fraction of resinous exudate of Heliotropium subulatum for their antioxidant antineoplastic and cytotoxic activities as well as to search new antioxidant and antineoplastic agents for pharmaceutical formulations Five flavonoids were isolated from resinous exudate of this plant species and screened for their in vitro and in vivo antioxidant models DPPH radical scavenging reducing power superoxide anion scavenging metal chelating scavenging systems catalase and lipid peroxidation antineoplastic Sarcoma 180 and cytotoxic Chinese hamster V79 cells activities Tricetin demonstrated maximum antioxidant activity against both in vitro and in vivo experimental systems while galangin exhibited maximum inhibition 7835 at a dose of 10 µg/kg/day against Sarcoma 180 Similarly it was found that galangin also showed highest activity 211 ± 015 at a concentration of 70 µg/ml to Chinese hamster V79 cells The observed results suggest that tricetin has a potential to scavenge free radicals in both in vitro and in vivo models while the galangin could be considered as antitumor and cytotoxic agentBS and PMS designed and perform the experimental work and analyzed the data RAS reviewed the manuscript and facilitated the help in equipments and chemicals We are grateful to Professor P Singh Department of Chemistry University of Rajasthan Jaipur for providing the standard compounds and Professor Dr H Itokawa College of Pharmacy Tokyo for his kind help in the screening process of antineoplastic and cytotoxic activities as well as Dr H Singh Pacific University for helping in study of in vivo antioxidant activities
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