Authors: Wenjing Zhang Yan Lan Qilai Huang Zichun Hua
Publish Date: 2012/09/22
Volume: 65, Issue: 3, Pages: 447-455
Abstract
Galangin an active flavonoid present at high concentration in Alpinia officinarum Hance and propolis shows cytotoxicity towards several cancer cell lines including melanoma However the specific cellular targets of galangininduced cytotoxicity in melanoma are still unknown Here we investigated the effects of galangin in B16F10 melanoma cells and explored the possible molecular mechanisms Galangin significantly decreased cell viability of B16F10 cells and also induced cell apoptosis shown by Hoechst 33342 staining and Annexin VPI double staining flow cytometric assay Furthermore upon galangin treatment disruption of mitochondrial membrane potential was observed by JC1 staining Western blotting analysis indicated that galangin activated apoptosis signaling cascades by cleavage of procaspase9 procaspase3 and PARP in B16F10 cells Moreover galangin significantly induced activation of phosphorp38 MAPK in a time and dose dependent manner SB203580 an inhibitor of p38 partially attenuated galangininduced apoptosis in B16F10 cells Taken together this work suggests that galangin has the potential to be a promising agent for melanoma treatment and may be further evaluated as a chemotherapeutic agentThis study was supported in part by the Science and Technology Development Fund of the Macao Special Administrative Region 071/2009/A3 and 091/2009/A the National Key Basic Research Project from Chinese Ministry of Science and Technology 2012CB967004 the Chinese National Nature Sciences Foundation 81121062 50973046 31070706 the Jiangsu Provincial Nature Science Foundation BK2010046 BZ2010074 BZ2011048 BK2011228 Bureau of Science and Technology of Changzhou CN20100016 CZ20100008 CE20115034 CZ20110028
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