Authors: Ece YildizOzturk Sultan GulceIz Muge Anil Ozlem YesilCeliktas
Publish Date: 2017/02/13
Volume: 69, Issue: 2, Pages: 337-347
Abstract
Two dimensional 2D cell culture systems lack the ability to mimic in vivo conditions resulting in limitations for preclinical cellbased drug and toxicity screening assays and modelling tumor biology Alternatively 3D cell culture systems mimic the specificity of native tissue with better physiological integrity In this regard microfluidic chips have gained wide applicability for in vitro 3D cancer cell studies The aim of this research was to develop a 3D biomimetic model comprising culture of breast cancer cells in butterflyshaped microchip to determine the cytotoxicity of carnosic acid and doxorubicin on both estrogen dependent MCF7 and independent MDAMB231 breast cancer cells along with healthy mammary epithelial cells MCF10A in 2D 3D Matrigel™ and butterflyshaped microchip environment According to the developed mimetic model carnosic acid exhibited a higher cytotoxicity towards MDAMB 231 while doxorubicin was more effective against MCF7 Although the cell viabilities were higher in comparison to 2D and 3D cell culture systems the responses of the investigated molecules were different in the microchips based on the molecular weight and structural complexity indicating the importance of biomimicry in a physiologically relevant matrix
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