Authors: Wenjun Wu Xinghua Gao Xianxiang Xu Yubin Luo Mei Liu Yufeng Xia Yue Dai
Publish Date: 2012/07/22
Volume: 65, Issue: 2, Pages: 287-295
Abstract
Our previous study reported that the saponinrich fraction from Clematis chinensis Osbeck roots SFC could effectively alleviate experimental osteoarthritis induced by monosodium iodoacetate in rats through protecting articular cartilage and inhibiting local inflammation The present study was performed to investigate the preventive effects of SFC on articular chondrocyte and explore the underlying mechanisms Primary rabbit chondrocytes were cultured and exposed to sodium nitroprusside SNP a NO donor After treatment with different concentrations of SFC 30 100 300 1000 μg/ml for 24 h nucleic morphology apoptotic rate mitochondrial function and caspase3 activity of chondrocytes were examined The results showed that SNP induced remarkable apoptosis of rabbit chondrocytes evidenced by Hoechst 33258 staining and flow cytometry analysis and SFC prevented the apoptosis in a concentrationdependent manner Further studies indicated that SFC could prevent the depolarization of mitochondrial membrane potential ∆ψm in SNPtreated chondrocytes and suppress the activation of caspase3 It can be concluded that the protection of SFC on articular chondrocytes is associated with the antiapoptosis effects via inhibiting the mitochondrion impairment and caspase3 activation
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