Authors: Tomoya Kawakami Atsushi Koike Fumio Amano
Publish Date: 2017/03/01
Volume: 69, Issue: 4, Pages: 631-642
Abstract
The role of activated macrophages in the host defense against pathogens or tumor cells has been investigated extensively Many researchers have been using various culture media in in vitro experiments using macrophages We previously reported that J7741/JA4 macrophagelike cells showed great differences in their activated macrophage phenotypes such as production of reactive oxygen nitric oxide NO or cytokines depending on the culture medium used either F12 Ham’s F12 nutrient mixture or Dulbecco modified Eagle’s medium DMEM To examine whether a difference in the culture medium would influence the functions of primary macrophages we used BALB/c mouse peritoneal macrophages in this study Among the activated macrophage phenotypes the expression of inducible NO synthase in LPS and/or IFNγtreated peritoneal macrophages showed the most remarkable differences between F12 and DMEM ie NO production by LPS and/or IFNγtreated cells was far lower in DMEM than in F12 Similar results were obtained with C57BL mouse peritoneal macrophages Besides dilution of F12 medium with saline resulted in a slight decrease in NO production whereas that of DMEM with saline resulted in a significant increase suggesting the possibility that DMEM contained some inhibitory factors for NO production However such a difference in NO production was not observed when macrophagelike cell lines were examined These results suggest that phenotypes of primary macrophages could be changed significantly with respect to host inflammatory responses by the surrounding environment including nutritional factors and that these altered macrophage phenotypes might influence the biological host defenseWe acknowledge Dr Ko Fujimori Osaka University of Pharmaceutical Sciences for his valuable discussion This work was supported in part by a GrantinAid for the Promotion of Science from the Ministry of Education Culture Sports Science and Technology of Japan JAPS KAKENHI Grant Number 24590165
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