Authors: SongLing Zhang YiShu Wang Tong Zhou XiaoWei Yu ZhenTong Wei YuLin Li
Publish Date: 2012/03/20
Volume: 64, Issue: 4, Pages: 477-484
Abstract
Cervical cancer is one of the most common gynecologic malignancies and poses a serious health problem worldwide Identification and characterization of cervical cancer stem cells may facilitate the development of novel strategies for the treatment of advanced and metastatic cervical cancer Breast cancerresistance protein Bcrp1positive cells were selected from a population of parent HeLa cells using flow cytometry The invasion capacity of Bcrp1positive and negative cells was analyzed with a Boyden chamber invasion test The tumorigenicity of these cells was determined by in vivo transplantation in nonobesity diabetes/severe combined immunodeficiency NOD/SCID mice The Bcrp1positive subpopulation accounted for about 7 of the parent HeLa cell population The proliferative capacity of the Bcrp1positive cells was greater than that of the Bcrp1negative cells P 005 In the invasion assay the Bcrp1positive cells demonstrated a greater invasive capacity through the artificial basement membrane than their Bcrp1negative counterparts Following transplantation of 104 cells only the Bcrp1positive cells formed tumors in NOD/SCID mice When 105 or 106 cells were transplanted the tumor incidence and the tumor mass were greater in the Bcrp1positive groups than those in the Bcrp1negative groups P 005 The Bcrp1positive subpopulation cervical cancer stem cells
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